Change search
ReferencesLink to record
Permanent link

Direct link
Chitinase genes revealed and compared in bacterial isolates, DNA extracts and a metagenomic library from a phytopathogen-suppressive soil
Södertörn University, School of Life Sciences, Molecular biology.
Show others and affiliations
2010 (English)In: FEMS Microbiology Ecology, ISSN 0168-6496, E-ISSN 1574-6941, Vol. 71, no 2, 197-207 p.Article in journal (Refereed) Published
Abstract [en]

Soil that is suppressive to disease caused by fungal pathogens is an interesting source to target for novel chitinases that might be contributing towards disease suppression. In this study, we screened for chitinase genes, in a phytopathogen-suppressive soil in three ways: (1) from a metagenomic library constructed from microbial cells extracted from soil, (2) from directly extracted DNA and (3) from bacterial isolates with antifungal and chitinase activities. Terminal restriction fragment length polymorphism (T-RFLP) of chitinase genes revealed differences in amplified chitinase genes from the metagenomic library and the directly extracted DNA, but approximately 40% of the identified chitinase terminal restriction fragments (TRFs) were found in both sources. All of the chitinase TRFs from the isolates were matched to TRFs in the directly extracted DNA and the metagenomic library. The most abundant chitinase TRF in the soil DNA and the metagenomic library corresponded to the TRF103 of the isolate Streptomyces mutomycini and/or Streptomyces clavifer. There were good matches between T-RFLP profiles of chitinase gene fragments obtained from different sources of DNA. However, there were also differences in both the chitinase and the 16S rRNA gene T-RFLP patterns depending on the source of DNA, emphasizing the lack of complete coverage of the gene diversity by any of the approaches used.

Place, publisher, year, edition, pages
United Kingdom: Wiley-Blackwell Publishing Ltd. , 2010. Vol. 71, no 2, 197-207 p.
Keyword [en]
metagenomic library, chitinase, terminal restriction fragment length polymorphism (T-RFLP), Streptomycetes, suppressive soil
National Category
Biological Sciences
Research subject
Environmental Studies
URN: urn:nbn:se:sh:diva-6179DOI: 10.1111/j.1574-6941.2009.00801.xISI: 000273065000003ScopusID: 2-s2.0-72949101011OAI: diva2:397113
Available from: 2011-02-11 Created: 2011-02-11 Last updated: 2016-10-07Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textScopus

Search in DiVA

By author/editor
Hjort, KarinJansson, Janet K.Sjöling, Sara
By organisation
Molecular biologyEnvironmental science
In the same journal
FEMS Microbiology Ecology
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 158 hits
ReferencesLink to record
Permanent link

Direct link