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The Interdomain Region of Dengue NS5 ProteinInteracts with NS3 and Host Proteins
Södertörn University College, Avdelning Naturvetenskap.
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2002 (English)In: Dengue Bulletin, ISSN 1020-895X, Vol. 26, 155-161 p.Article in journal (Other academic) Published
Abstract [en]

Although dengue virus genome replication occurs in the cytoplasm of infected cells, it has been shown that the NS5 protein (RNA-dependent RNA polymerase) is hyperphosphorylated at a late stage in infection and localized to the cell nucleus. A 37 amino acid sequence of NS5 (residues 369-405) was shown to contain a functional nuclear localization signal (NLS) that interacted with the cellular nuclear transport factor, importin α/β heterodimer. Further studies using the yeast two-hybrid system revealed that the NS5 region (residues 320-368) immediately adjacent to the NLS contained an importin β-binding site that abuts or overlaps the binding site for the NS3 protein (protease/helicase). The importin β-binding site has also been shown to be a functional NLS (bNLS). Intriguingly, when both bNLS and NLS (residues 320-405) were present, the fused β -galactosidase protein did not accumulate in the nucleus. Here we provide a review of our studies on the NS5 interdomain region and compare it to other members of the Flavivirus genus in order to highlight the importance of this region as a possible target for developing broad-acting antiviral agent against dengue and other mechanistically-related viruses.

Place, publisher, year, edition, pages
2002. Vol. 26, 155-161 p.
Keyword [en]
NS5 protein, protein interactions, NS3 protein, nuclear localization signal
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:sh:diva-6066OAI: oai:DiVA.org:sh-6066DiVA: diva2:395555
Available from: 2011-02-07 Created: 2011-02-07Bibliographically approved

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