Change search
ReferencesLink to record
Permanent link

Direct link
The Interdomain Region of Dengue NS5 ProteinInteracts with NS3 and Host Proteins
Södertörn University College, Avdelning Naturvetenskap.
Show others and affiliations
2002 (English)In: Dengue Bulletin, ISSN 1020-895X, Vol. 26, 155-161 p.Article in journal (Other academic) Published
Abstract [en]

Although dengue virus genome replication occurs in the cytoplasm of infected cells, it has been shown that the NS5 protein (RNA-dependent RNA polymerase) is hyperphosphorylated at a late stage in infection and localized to the cell nucleus. A 37 amino acid sequence of NS5 (residues 369-405) was shown to contain a functional nuclear localization signal (NLS) that interacted with the cellular nuclear transport factor, importin α/β heterodimer. Further studies using the yeast two-hybrid system revealed that the NS5 region (residues 320-368) immediately adjacent to the NLS contained an importin β-binding site that abuts or overlaps the binding site for the NS3 protein (protease/helicase). The importin β-binding site has also been shown to be a functional NLS (bNLS). Intriguingly, when both bNLS and NLS (residues 320-405) were present, the fused β -galactosidase protein did not accumulate in the nucleus. Here we provide a review of our studies on the NS5 interdomain region and compare it to other members of the Flavivirus genus in order to highlight the importance of this region as a possible target for developing broad-acting antiviral agent against dengue and other mechanistically-related viruses.

Place, publisher, year, edition, pages
2002. Vol. 26, 155-161 p.
Keyword [en]
NS5 protein, protein interactions, NS3 protein, nuclear localization signal
National Category
Medical and Health Sciences
URN: urn:nbn:se:sh:diva-6066OAI: diva2:395555
Available from: 2011-02-07 Created: 2011-02-07Bibliographically approved

Open Access in DiVA

No full text

Other links

Search in DiVA

By author/editor
Johansson, Magnus
By organisation
Avdelning Naturvetenskap
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar

Total: 84 hits
ReferencesLink to record
Permanent link

Direct link