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Effects of copper on olfactory-mediated endocrine responses and reproductive behaviour in mature male brown trout Salmo trutta parr to conspecific females
Södertörn University, School of Life Sciences, Environmental science. Södertörn University, School of Life Sciences, Biology.
2010 (English)In: Journal of Fish Biology, ISSN 0022-1112, E-ISSN 1095-8649, Vol. 76, no 4, 800-817 p.Article in journal (Refereed) Published
Abstract [en]

In the present study, the effects of copper (CuSO4) on the ability of mature male brown trout Salmo trutta parr to detect and react both physiologically and behaviourally to female pheromones were studied. The study was composed of two parts. In the first experiment, priming effects of the female pheromone prostaglandin F-2 alpha (PGF(2 alpha)) were evaluated by determining the amount of milt produced and the blood plasma levels of 11-ketotestosterone (11-KT) and 17 alpha,20 beta-dihydroxy-4-pregnen-3-one (17,20 beta-P) after the PGF(2 alpha) exposure. In the second experiment, male parr were placed in a large stream tank together with a group of adult males and ovulated females and their individual behaviours were recorded. In the priming experiment, the amount of expressible milt was significantly lower, less than half, in groups exposed during 4 days to 10 or 100 mu g l-1 copper compared with control parr only exposed to water. No significant differences were observed in plasma levels of 11-KT and 17, 20 beta-P. During the behavioural experiment, exposed parr spent less time with the female and had a lower number of courting events. Blood plasma levels of 11-KT were, however, significantly higher in the group exposed to 100 mu g l-1 copper compared with the control group. Furthermore, the exposed group spent significantly less time swimming upstream than did the control group. The present study demonstrates that exposure to copper affects reproductive behaviours and endocrinology of S. trutta male parr.

Place, publisher, year, edition, pages
2010. Vol. 76, no 4, 800-817 p.
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:sh:diva-5835DOI: 10.1111/j.1095-8649.2009.02519.xISI: 000275466200003ScopusID: 2-s2.0-77951657749OAI: oai:DiVA.org:sh-5835DiVA: diva2:393735
Available from: 2011-02-01 Created: 2011-01-31 Last updated: 2014-02-13Bibliographically approved
In thesis
1. Pheromonal Mediated Behaviour and Endocrine Responses in Salmonids: The impact of Cypermethrin, Copper, and Glyphosate
Open this publication in new window or tab >>Pheromonal Mediated Behaviour and Endocrine Responses in Salmonids: The impact of Cypermethrin, Copper, and Glyphosate
2010 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The effects of cypermethrin, copper and glyphosate on the endocrine system and subsequent response to female pheromones were investigated in mature male brown trout (Salmo trutta) parr.  Responses measured were the amount of strippable milt, blood plasma levels of both an androgen (11-ketotestosterone (11-KT)) and a progestin (17α,20β-dihydroxy-4-pregnen-3-one (17,20b-P)), and behavioural changes. This was done in a two phased investigation where parr were exposed to one of the following via ambient water: 1) 0.1 or 1.0 μg L-1 cypermethrin, 2) 10 or 100 μg L-1 copper (Cu2+), or 3) 150 μg L-1 glyphosate for a 96 hour period.  Phase one was a priming experiment exposing parr to a treatment followed by priming with PGF or ovarian fluid (OVF). Atlantic salmon (Salmo salar) parr were, also exposed to glyphosate during phase I. The second phase was centered on behavioural observations.  Exposed parr were placed in a 35,000 L stream aquarium together with two ovulated females and four anadromous males. After the experiments a blood sample was taken, milt volumes measured and testes weighed.  The plasma was analyzed for 11-KT and 17,20b-P concentrations using radioimmunoassay (RIA).

Results from phase I-priming: 1.0 μg L-1 cypermethrin exposure lowered 17,20b-P and 11-KT; Copper exposure lowered milt volumes; glyphosate exposure lowered 11-KT in salmon and raised 17,20b-P in trout.  Results from phase II-behaviour: 1.0 μg L-1 cypermethrin exposure lowered 11-KT, milt and spawning behaviour; copper exposure lowered spawning behaviour and raised 11-KT; Glyphosate exposure lowered 11KT; continuous cypermethrin exposure raised 17,20b-P, 11-KT and gave a tendency towards increased aggression. It is concluded that low concentration exposure to the compounds examined can induce negative effects on male salmonid endocrine systems, either through a disruption in the olfactory system or through a direct effect.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2010. 52 p.
Series
Södertörn Doctoral Dissertations, ISSN 1652-7399 ; 46
Series
, Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 730
Keyword
Cypermethrin, copper, glyphosate, behaviour, hormones, pheromones, olfaction, 11-ketotestosterone (11-KT), 17α, 20β-dihydroxy-4-pregnen-3-one (17, 20β-P), reproduction
National Category
Pharmacology and Toxicology
Research subject
Biology with specialization in Environmental Toxicology
Identifiers
urn:nbn:se:sh:diva-14761 (URN)978-91-554-7772-1 (ISBN)
Public defence
2010-05-07, Lindahlsalen, Evolutionsbiologiskt centrum, Norbyvägen 18A, 09:00 (English)
Opponent
Supervisors
Available from: 2012-01-16 Created: 2012-01-16 Last updated: 2012-11-01Bibliographically approved

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