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Developing electroporation as a method to obtain Stable Transformation in Drosophila melanogaster
Södertörn University College, School of Life Sciences. (Drosophila lab.)
2008 (English)Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

In this project I have tried to obtain stable transformants of Drosophila melanogaster flies using electroporation. I have completed approximately 200 tests using different DNA concentrations, voltages and cuvettes, including a novel Petri dish cuvette which I developed and manufactured myself. I also developed new and more efficient procedures of egg collection and egg dechorionation. Although I was not  successful in obtaining true stable transformants, control experiments indicate that electroporation of DNA into embryos could be accomplished under the conditions used. The lack of stable transformants was probably due to failure of the electroporated DNA to integrate into the host genome. The reasons for why the DNA did not integrate was not further investigated in this study.

Place, publisher, year, edition, pages
2008. , p. 28
Keywords [en]
Stable Transformation, electroporation, Drosophila melanogaster
National Category
Developmental Biology
Identifiers
URN: urn:nbn:se:sh:diva-3245OAI: oai:DiVA.org:sh-3245DiVA, id: diva2:299663
Presentation
(English)
Uppsok
Life Earth Science
Supervisors
Examiners
Available from: 2010-02-23 Created: 2010-02-23 Last updated: 2010-02-24Bibliographically approved

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CiteExportLink to record
Permanent link

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Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • harvard-anglia-ruskin-university
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf