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The HMG box of the histone lysine methylase spLsd1 is required for entry into quiescence
Södertörn University College, School of Life Sciences. (Karl Ekwall)
2008 (English)Independent thesis Advanced level (degree of Magister), 20 poäng / 30 hpStudent thesis
Abstract [en]

The capability to control the progression of the cell cycle, including the means to enter into a stable non-proliferative state, is essential for eukaryotic unicellular and multicellular organisms. A quiescent state similar to G0 of higher eukaryotes can be induced by nitrogen starvation of the fission yeast model organism Schizosaccharomyces pombe. Using high-resolution tiling arrays for genome-wide transcriptional profiling we explore the early transcriptional reprogramming on the route to quiescence. Furthermore, we demonstrate that cells carrying a mutation in the high mobility group (HMG) box of the histone lysine demethylase spLsd1 fail to acquire characteristics of quiescent cells and rapidly lose viability under nitrogen-starved conditions. Since no such defect is seen as a result of catalytic inactivation, the HMG domain of spLsd1 seems to confer a function to the protein that is independent of the histone demethylase activity. We show that the HMG domain of spLsd1 is required for transcriptional activation and repression of a large set of genes, both during vegetative growth and on the route to quiescence. We also confirm that spLsd1 is a repressor of antisense transcription, and that this function is at least partially dependent on the HMG domain of the protein.

Place, publisher, year, edition, pages
2008. , 39 p.
Keyword
Lsd1, pombe, quiescence, transcription
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:sh:diva-1772OAI: oai:DiVA.org:sh-1772DiVA: diva2:224
Uppsok
bio-/geovetenskap
Supervisors
Examiners
Available from: 2008-09-09 Created: 2008-09-08 Last updated: 2008-09-09Bibliographically approved

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CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf