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Analysis of gene expression in barley upon aphid attack
Södertörn University College, School of Life Sciences.
2007 (English)Independent thesis Basic level (professional degree), 20 points / 30 hpStudent thesis
Abstract [en]

Since plants can not escape their predators by walking, they use some other defense systems, like induction or repression of defense genes. A microarray experiment performed with barley attacked by the bird cherry-oat aphid (Rhopalosiphum padi), led to the hypothesis that contig 16360 (similar to ser/thr kinases) could be linked with the resistance of barley against R. padi, and contig 6519 (similar to WIR 1A) with the susceptibility. Time course experiments showed that contig16360 and AJ250283 (similar to BCI-4) are almost induced in the same way, each, by two different aphids (R. padi and Metopolophium dirhodum). Genomic PCR was used to test the hypothesis that when plants have the gene for contig 16360, they are more likely to be resistant against aphid attack, and when plants have the gene for contig 6519, they are more likely to be susceptible. This test was performed with 69 barley lines: wild, commercial or breeding lines. Results were that the presence of WIR 1A gene has no correlation with the susceptibility, while presence of ser/thr kinase seems to be correlated with resistance.

Place, publisher, year, edition, pages
2007. , 25 p.
Keyword [en]
aphids, barley, R. padi, defense reactions, gene regulation, genomic PCR
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:sh:diva-1289OAI: oai:DiVA.org:sh-1289DiVA: diva2:15353
Uppsok
bio-/geovetenskap
Supervisors
Examiners
Available from: 2007-07-04 Created: 2007-07-04

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CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf