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Noninvasive monitoring of apoptosis versus necrosis in a neuroblastoma cell line expressing a nuclear pore protein tagged with the green fluorescent protein
Stockhlms univeristet.
Stockholms universitet.
Stockholms universitet.
Stockholms universitet.
1998 (English)In: Experimental Cell Research, ISSN 0014-4827, E-ISSN 1090-2422, Vol. 238, no 2, 371-376 p.Article in journal (Refereed) Published
Abstract [en]

A fusion chimera between the integral nuclear pore membrane protein POM121 and GFP (green fluorescent protein) has been shown to correctly target to the nuclear pores when transiently expressed in a number of mammalian cell types. POM121-GFP is therefore an excellent marker for the noninvasive studies of the nuclear pores in living cells using fluorescence microscopy. We have established a line of neuroblastoma cells stably expressing the POM121-GFP fusion protein. We also monitored the nuclear envelope in living cells after induction of apoptosis or necrosis using 1 μM staurosporine or 100 μM p-benzoquinone, respectively. Interestingly, the POM121-GFP fluorescence was weaker or missing in the apoptotic cells. The disappearance of the nuclear pore marker accompanied apoptotic progression as judged by the degree of chromatin condensation and DNA fragmentation as analyzed by DNA staining and TUNEL assay, respectively. In contrast, the intensity of the nuclear rim fluorescence was unaffected in necrotic cells displaying an abnormal morphology with tilted nuclei. Thus, it was possible to distinguish between apoptotic and necrotic development in living cells using fluorescence microscopy. This cell line provides a fast and convenient model for screening suspected toxic xenobiotics.

Place, publisher, year, edition, pages
1998. Vol. 238, no 2, 371-376 p.
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:sh:diva-31520DOI: 10.1006/excr.1997.3846Scopus ID: 2-s2.0-0032004728OAI: oai:DiVA.org:sh-31520DiVA: diva2:1059644
Available from: 2016-12-22 Created: 2016-12-22 Last updated: 2016-12-22Bibliographically approved
In thesis
1. Distribution and dynamics of a nuclear pore membrane protein
Open this publication in new window or tab >>Distribution and dynamics of a nuclear pore membrane protein
2002 (English)Doctoral thesis, comprehensive summary (Other academic)
Place, publisher, year, edition, pages
Stockholm: Stockholms universitet, 2002. 42 p.
Keyword
Proteiner
National Category
Biological Sciences
Identifiers
urn:nbn:se:sh:diva-31517 (URN)91-7265-391-4 (ISBN)
Public defence
2002-02-08, Magnélisalen, Kemiska Övningslaboratoriet, Svante Arrheniusväg 12, Stockholm, 10:00
Opponent
Supervisors
Available from: 2016-12-22 Created: 2016-12-22 Last updated: 2016-12-22Bibliographically approved

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CiteExportLink to record
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