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Intracellular distribution of an integral nuclear pore membrane protein fused to green fluorescent protein - Localization of a targeting domain
Stockhlms univeristet.
Stockholms universitet.
Stockholms universitet.
Karolinska institutet.
Show others and affiliations
1997 (English)In: European Journal of Biochemistry, ISSN 0014-2956, E-ISSN 1432-1033, Vol. 250, no 3, 808-813 p.Article in journal (Refereed) Published
Abstract [en]

The 121-kDa pore membrane protein (POM121) is a bitopic integral membrane protein specifically located in the pore membrane domain of the nuclear envelope with its short N-terminal tail exposed on the luminal side and its major C-terminal portion adjoining the nuclear pore complex, In order to locate a signal for targeting of POM121 to the nuclear pores, we overexpressed selected regions of POM121 alone or fused to the green fluorescent protein (GFP) in transiently transfected COS-1 cells or in a stably transfected neuroblastoma cell line. Microscopic analysis of the GFP fluorescence or immunostaining was used to determine the intracellular distribution of the overexpressed proteins. The endofluorescent GFP tag had no effect on the distribution of POM121, since the chimerical POM121-GFP fusion protein was correctly targeted to the nuclear ports of both COS-1 cells and neuroblastoma cells. Based on the differentiated intracellular sorting of the POM121 variants, we conclude that the first 128 amino acids of POM121 contains signals for targeting to the continuous endoplasmic reticulum/nuclear envelope membrane system but not specifically to the nuclear pol es and that a specific nuclear pore targeting signal is located between amino acids 129 and 618 in the endoplasmically exposed portion of POM121.

Place, publisher, year, edition, pages
1997. Vol. 250, no 3, 808-813 p.
National Category
Biological Sciences
Identifiers
URN: urn:nbn:se:sh:diva-31518DOI: 10.1111/j.1432-1033.1997.00808.xISI: 000071370300024PubMedID: 9461306OAI: oai:DiVA.org:sh-31518DiVA: diva2:1059636
Available from: 2016-12-22 Created: 2016-12-22 Last updated: 2016-12-22Bibliographically approved
In thesis
1. Distribution and dynamics of a nuclear pore membrane protein
Open this publication in new window or tab >>Distribution and dynamics of a nuclear pore membrane protein
2002 (English)Doctoral thesis, comprehensive summary (Other academic)
Place, publisher, year, edition, pages
Stockholm: Stockholms universitet, 2002. 42 p.
Keyword
Proteiner
National Category
Biological Sciences
Identifiers
urn:nbn:se:sh:diva-31517 (URN)91-7265-391-4 (ISBN)
Public defence
2002-02-08, Magnélisalen, Kemiska Övningslaboratoriet, Svante Arrheniusväg 12, Stockholm, 10:00
Opponent
Supervisors
Available from: 2016-12-22 Created: 2016-12-22 Last updated: 2016-12-22Bibliographically approved
2. Targeting of a nascent integral membrane protein to the nuclear pores and its degradation during apoptosis
Open this publication in new window or tab >>Targeting of a nascent integral membrane protein to the nuclear pores and its degradation during apoptosis
2002 (English)Doctoral thesis, comprehensive summary (Other academic)
Place, publisher, year, edition, pages
Stockholm: Stockholms universitet, 2002. 46 p.
National Category
Biological Sciences
Identifiers
urn:nbn:se:sh:diva-31521 (URN)91-7265-559-3 (ISBN)
Public defence
2002-12-13, Magnélisalen, Kemiska Övningslaboratoriet, Svante Arrenihusväg 12, Stockholm, 10:00
Opponent
Supervisors
Available from: 2016-12-22 Created: 2016-12-22 Last updated: 2016-12-22Bibliographically approved

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CiteExportLink to record
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Citation style
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