Transient inhibition of histone deacetylase activity overcomes silencing in the mating-type region in fission yeast
1999 (English)In: Current Genetics, ISSN 0172-8083, E-ISSN 1432-0983, Vol. 35, no 2, 82-87 p.Article in journal (Refereed) Published
We have investigated the effects of inhibition of histone de-acetylase activity on silencing at the silent mating-type loci in fission yeast. Treatment of exponentially growing cells with the histone deacetylase inhibitor, trichostatin A (TSA), resulted in derepression of a marker gene inserted 150 bp distal from the silent mat3-M locus. The natural targets for the silencing mechanism in this region were only partially derepressed and the activation appeared to be asymmetric. i.e. the mat2-P cassette remained silent at concentrations that clearly partially derepressed the mat3-M cassette. We further noted that treatment of wild-type h(90) cells resulted in the generation of altered sporulation phenotypes, indicating that the treatment affected the expression of mating-type genes and/or mating-type switching. The results are discussed in the light of recent accumulated data regarding the role of deacetylation for silencing in other species.
Place, publisher, year, edition, pages
1999. Vol. 35, no 2, 82-87 p.
trichostatin A, repression, Schizosaccharomyces pombe, heterochromatin
IdentifiersURN: urn:nbn:se:sh:diva-31263DOI: 10.1007/s002940050436ISI: 000079273500004PubMedID: 10079326ScopusID: 2-s2.0-0033061968OAI: oai:DiVA.org:sh-31263DiVA: diva2:1051213