sh.sePublikasjoner
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • harvard-anglia-ruskin-university
  • apa-old-doi-prefix.csl
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Scribble controls NGF-mediated neurite outgrowth in PC12 cells
Södertörns högskola, Institutionen för naturvetenskap, miljö och teknik, Biologi. Dalhousie University.
Södertörns högskola, Institutionen för naturvetenskap, miljö och teknik, Biologi.ORCID-id: 0000-0003-4442-8503
Södertörns högskola, Institutionen för naturvetenskap, miljö och teknik, Biologi.
Södertörns högskola, Institutionen för naturvetenskap, miljö och teknik, Biologi. Örebro university.
2013 (engelsk)Inngår i: European Journal of Cell Biology, ISSN 0171-9335, E-ISSN 1618-1298, Vol. 92, nr 6-7, s. 213-221Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Neurite outgrowth is mediated by dynamic changes of the cytoskeleton and is largely controlled by Rho GTPases and their regulators. Here, we show that the polarity protein Scribble controls PC12 cell neurite outgrowth in response to nerve growth factor. Scribble knockdown decreases neurite numbers and increases neurite length. This effect is linked to TrkA the cognate receptor for NGF as pharmacological inhibition of phosphorylated TrkA (pTrkA) reduces Scribble expression. Moreover, Scribble forms a complex with the MAPK components ERK1/2 in a growth factor dependent manner. In RNAi experiments where Scribble expression is efficiently depleted sustained ERK1/2 phosphorylation is reduced. Conversely, siRNA with intermediate Scribble silencing efficiency fails to match this effect indicating that ERK1/2 activation depends on basic Scribble protein levels. Finally, Scribble translocates to the plasma membrane in response to growth factor where it complexes with HRas and Rac1 suggesting that the phenotype activated by loss of Scribble may be a result of altered GTPase activity. Together, these results demonstrate a novel role for Scribble in neurite outgrowth of PC12 cells.

sted, utgiver, år, opplag, sider
2013. Vol. 92, nr 6-7, s. 213-221
HSV kategori
Identifikatorer
URN: urn:nbn:se:sh:diva-19833DOI: 10.1016/j.ejcb.2013.07.002ISI: 000324971200003PubMedID: 23973368Scopus ID: 2-s2.0-84883244028OAI: oai:DiVA.org:sh-19833DiVA, id: diva2:651890
Forskningsfinansiär
The Foundation for Baltic and East European StudiesKnowledge Foundation
Merknad

Funding agency: Carl Tryggers foundation

Tilgjengelig fra: 2013-09-27 Laget: 2013-09-27 Sist oppdatert: 2017-12-06bibliografisk kontrollert

Open Access i DiVA

Fulltekst mangler i DiVA

Andre lenker

Forlagets fulltekstPubMedScopus

Personposter BETA

Wigerius, MichaelAsghar, NaveedMelik, WessamJohansson, Magnus

Søk i DiVA

Av forfatter/redaktør
Wigerius, MichaelAsghar, NaveedMelik, WessamJohansson, Magnus
Av organisasjonen
I samme tidsskrift
European Journal of Cell Biology

Søk utenfor DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric

doi
pubmed
urn-nbn
Totalt: 186 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • harvard-anglia-ruskin-university
  • apa-old-doi-prefix.csl
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf