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The arginine methyltransferase Rmt2 is enriched in the nucleus and co-purifies with the nuclear porins Nup49, Nup57 and Nup100
Södertörns högskola, Institutionen för livsvetenskaper.
Södertörns högskola, Institutionen för livsvetenskaper.
CHORI, Children's Hospital Oakland Research Institute, Oakland, USA / Umeå University.
Columbia University, New York, USA.
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2007 (Engelska)Ingår i: Experimental Cell Research, ISSN 0014-4827, E-ISSN 1090-2422, Vol. 313, nr 9, s. 1778-1789Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Arginine methylation is a post-translational modification of proteins implicated in RNA processing, protein compartmentalization, signal transduction, transcriptional regulation and DNA repair. In a screen for proteins associated with the nuclear envelope in the yeast Saccharomyces cerevisiae, we have identified the arginine methyltransferase Rmt2, previously shown to methylate the ribosomal protein L12. By indirect immunofluorescence and subcellular fractionations we demonstrate here that Rmt2 has nuclear and cytoplasmic localizations. Biochemical analysis of a fraction enriched in nuclei reveals that nuclear Rmt2 is resistant to extractions with salt and detergent, indicating an association with structural components. This was supported by affinity purification experiments with TAP-tagged Rmt2. Rmt2 was found to co-purify with the nucleoporins Nup49, Nup57 and Nup100, revealing a novel link between arginine methyltransferases and the nuclear pore complex. In addition, a genome-wide transcription study of the rmt2 Delta mutant shows significant downregulation of the transcription of MYO1, encoding the Type II myosin heavy chain required for cytokinesis and cell separation.

Ort, förlag, år, upplaga, sidor
2007. Vol. 313, nr 9, s. 1778-1789
Nationell ämneskategori
Cancer och onkologi Cellbiologi
Identifikatorer
URN: urn:nbn:se:sh:diva-14224DOI: 10.1016/j.yexcr.2007.03.007ISI: 000246348400004PubMedID: 17448464Scopus ID: 2-s2.0-35548947153OAI: oai:DiVA.org:sh-14224DiVA, id: diva2:467810
Tillgänglig från: 2011-12-19 Skapad: 2011-12-19 Senast uppdaterad: 2017-12-08Bibliografiskt granskad
Ingår i avhandling
1. Functional studies of nuclear envelope-associated proteins in Saccharomyces cerevisiae
Öppna denna publikation i ny flik eller fönster >>Functional studies of nuclear envelope-associated proteins in Saccharomyces cerevisiae
2008 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Proteins of the nuclear envelope play important roles in a variety of cellular processes e.g. transport of proteins between the nucleus and cytoplasm, co-ordination of nuclear and cytoplasmic events, anchoring of chromatin to the nuclear periphery and regulation of transcription. Defects in proteins of the nuclear envelope and the nuclear pore complexes have been related to a number of human diseases. To understand the cellular functions in which nuclear envelope proteins participate it is crucial to map the functions of these proteins.

The present study was done in order to characterize the role of three different proteins in functions related to the nuclear envelope in the yeast Saccharomyces cerevisiae. The arginine methyltransferase Rmt2 was demonstrated to associate with proteins of the nuclear pore complexes and to influence nuclear export. In addition, Rmt2 was found to interact with the Lsm4 protein involved in RNA degradation, splicing and ribosome biosynthesis. These results provide support for a role of Rmt2 at the nuclear periphery and potentially in nuclear transport and RNA processing. The integral membrane protein Cwh43 was localized to the inner nuclear membrane and was also found at the nucleolus. A nuclear function for Cwh43 was demonstrated by its ability to bind DNA in vitro. A link to nucleolar functions was demonstrated by genetic analysis. Furthermore, Cwh43 is interacting with signalling pathways perhaps acting as a sensor for signals transmitted from the cytoplasm to the nucleus. The Myr1 protein was found to be membrane-associated and to interact with proteins involved in vesicular traffic. Overexpression of Myr1 affects nuclear morphology and nuclear pore distribution suggesting a function in membrane dynamics.

In conclusion, the presented results aid in a deeper understanding of functions related to the nuclear envelope in revealing a novel link between arginine methylation and the nuclear periphery, identifying a novel inner nuclear membrane protein and a new membrane-associated protein.

Ort, förlag, år, upplaga, sidor
Stockholm: Department of Biochemistry and Biophysics, Stockholm university, 2008. s. 58
Nyckelord
Nucleus, nuclear envelope, nuclear pore complexes, vesicular traffic, arginine methylation, Rmt2, Cwh43, Myr1
Nationell ämneskategori
Biokemi och molekylärbiologi
Identifikatorer
urn:nbn:se:sh:diva-32040 (URN)978-91-7155-666-0 (ISBN)
Disputation
2008-05-29, MA 636, Alfred Nobels allé 7, Huddinge, 13:00 (Engelska)
Opponent
Handledare
Tillgänglig från: 2017-02-13 Skapad: 2017-02-13 Senast uppdaterad: 2017-02-13Bibliografiskt granskad

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Olsson, IdaBerrez, Jean-MarcMutvei, Ann

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Experimental Cell Research
Cancer och onkologiCellbiologi

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