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Microbial community structure in polluted Baltic Sea sediments
Södertörns högskola, Institutionen för livsvetenskaper. SLU.
Södertörns högskola, Institutionen för livsvetenskaper.ORCID-id: 0000-0002-5802-5126
2006 (Engelska)Ingår i: Environmental Microbiology, ISSN 1462-2912, E-ISSN 1462-2920, Vol. 8, nr 2, s. 223-232Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Nearly half the seabed of the Baltic Proper is incapable of supporting life of higher organisms as a consequence of oxygen depletion resulting from eutrophication. However, these areas are actually teeming with microbial life. Here we used terminal-restriction fragment length polymorphism (T-RFLP) to investigate the dominant archaeal and bacterial groups, with respect to community structure, in surface layers of bottom sediments of the Baltic Sea along a coastal pollution gradient. Both archaeal and bacterial communities formed distinct clusters along the pollution gradient and the community compositions were different at the polluted sites compared with the relatively clean reference sites. The structures of the bacterial communities were most strongly correlated to water depth, followed by organic carbon, oxygen, salinity and silicate levels. In contrast, the structures of the archaeal communities were most strongly correlated to oxygen, salinity, organic carbon, silicate and nitrate levels. Some members of the microbial communities were identified using a combination of traditional and molecular approaches. Isolates obtained on different culture media were identified by partial sequencing of their 16S rRNA genes and some novel species were found. In addition, we developed a computer program, APLAUS, to elucidate the putative identities of the most dominant community members by T-RFLP.

Ort, förlag, år, upplaga, sidor
United Kingdom: Wiley-Blackwell Publishing Ltd. , 2006. Vol. 8, nr 2, s. 223-232
Nyckelord [en]
RIBOSOMAL-RNA; MARINE BACTERIOPLANKTON; DIVERSITY; EUTROPHICATION; ANTARCTICA; SALINITY; BACTERIAL; DYNAMICS; ARCHAEA; WATERS
Nationell ämneskategori
Naturvetenskap
Forskningsämne
Miljövetenskapliga studier
Identifikatorer
URN: urn:nbn:se:sh:diva-6194DOI: 10.1111/j.1462-2920.2005.00887.xISI: 000234647600005PubMedID: 16423011Scopus ID: 2-s2.0-33644870711OAI: oai:DiVA.org:sh-6194DiVA, id: diva2:397122
Tillgänglig från: 2011-02-11 Skapad: 2011-02-11 Senast uppdaterad: 2017-12-11Bibliografiskt granskad
Ingår i avhandling
1. Microbial diversity in Baltic Sea sediments
Öppna denna publikation i ny flik eller fönster >>Microbial diversity in Baltic Sea sediments
2007 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

This thesis focuses on microbial community structures and their functions in Baltic Sea sediments. First we investigated the distribution of archaea and bacteria in Baltic Sea sediments along a eutrophication gradient. Community profile analysis of 16S rRNA genes using terminal restriction length polymorphism (T-RFLP) indicated that archaeal and bacterial communities were spatially heterogeneous. By employing statistical ordination methods we observed that archaea and bacteria were structured and impacted differently by environmental parameters that were significantly linked to eutrophication. In a separate study, we analyzed bacterial communities at a different site in the Baltic Sea that was heavily contaminated with polyaromatic hydrocarbons (PAHs) and several other pollutants. Sediment samples were collected before and after remediation by dredging in two consecutive years. A polyphasic experimental approach was used to assess growing bacteria and degradation genes in the sediments. The bacterial communities were significantly different before and after dredging of the sediment. Several isolates collected from contaminated sediments showed an intrinsic capacity for degradation of phenanthrene (a PAH model compound). Quantititative real-time PCR was used to monitor the abundance of degradation genes in sediment microcosms spiked with phenanthrene. Although both xylE and phnAc genes increased in abundance in the microcosms, the isolates only carried phnAc genes. Isolates with closest 16S rRNA gene sequence matches to Exigobacterium oxidotolerans, a Pseudomonas sp. and a Gammaproteobacterium were identified by all approaches used as growing bacteria that are capable of phenanthrene degradation. These isolates were assigned species and strain designations as follows: Exiguobacterium oxidotolerans AE3, Pseudomonas fluorescens AE1 and Pseudomonas migulae AE2. We also identified and studied the distribution of actively growing bacteria along red-ox profiles in Baltic Sea sediments. Community structures were found to be significantly different at different red-ox depths. Also, according to multivariate statistical ordination analysis organic carbon, nitrogen, and red-ox potential were crucial parameters for structuring the bacterial communities on a vertical scale. Novel lineages of bacteria were obtained by sequencing 16S rRNA genes from different red-ox depths and sampling stations indicating that bacterial diversity in Baltic Sea sediments is largely unexplored.

Ort, förlag, år, upplaga, sidor
Uppsala: Sveriges Lantbruksuniversitet, 2007. s. 36
Serie
Acta Universitatis agriculturae Sueciae, ISSN 1652-6880 ; 2007:6
Nyckelord
Havsbottnen, Mikroorganismer
Nationell ämneskategori
Biologiska vetenskaper
Forskningsämne
Östersjö- och Östeuropaforskning
Identifikatorer
urn:nbn:se:sh:diva-31261 (URN)91-576-7325-X (ISBN)
Handledare
Tillgänglig från: 2016-12-01 Skapad: 2016-12-01 Senast uppdaterad: 2016-12-01Bibliografiskt granskad

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Edlund, AnnaSjöling, Sara

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