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Chitinase genes revealed and compared in bacterial isolates, DNA extracts and a metagenomic library from a phytopathogen-suppressive soil
Södertörns högskola, Institutionen för livsvetenskaper, Molekylärbiologi.
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2010 (Engelska)Ingår i: FEMS Microbiology Ecology, ISSN 0168-6496, E-ISSN 1574-6941, Vol. 71, nr 2, s. 197-207Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Soil that is suppressive to disease caused by fungal pathogens is an interesting source to target for novel chitinases that might be contributing towards disease suppression. In this study, we screened for chitinase genes, in a phytopathogen-suppressive soil in three ways: (1) from a metagenomic library constructed from microbial cells extracted from soil, (2) from directly extracted DNA and (3) from bacterial isolates with antifungal and chitinase activities. Terminal restriction fragment length polymorphism (T-RFLP) of chitinase genes revealed differences in amplified chitinase genes from the metagenomic library and the directly extracted DNA, but approximately 40% of the identified chitinase terminal restriction fragments (TRFs) were found in both sources. All of the chitinase TRFs from the isolates were matched to TRFs in the directly extracted DNA and the metagenomic library. The most abundant chitinase TRF in the soil DNA and the metagenomic library corresponded to the TRF103 of the isolate Streptomyces mutomycini and/or Streptomyces clavifer. There were good matches between T-RFLP profiles of chitinase gene fragments obtained from different sources of DNA. However, there were also differences in both the chitinase and the 16S rRNA gene T-RFLP patterns depending on the source of DNA, emphasizing the lack of complete coverage of the gene diversity by any of the approaches used.

Ort, förlag, år, upplaga, sidor
United Kingdom: Wiley-Blackwell Publishing Ltd. , 2010. Vol. 71, nr 2, s. 197-207
Nyckelord [en]
metagenomic library, chitinase, terminal restriction fragment length polymorphism (T-RFLP), Streptomycetes, suppressive soil
Nationell ämneskategori
Biologiska vetenskaper
Forskningsämne
Miljövetenskapliga studier
Identifikatorer
URN: urn:nbn:se:sh:diva-6179DOI: 10.1111/j.1574-6941.2009.00801.xISI: 000273065000003Scopus ID: 2-s2.0-72949101011OAI: oai:DiVA.org:sh-6179DiVA, id: diva2:397113
Tillgänglig från: 2011-02-11 Skapad: 2011-02-11 Senast uppdaterad: 2017-12-11Bibliografiskt granskad

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Hjort, KarinJansson, Janet K.Sjöling, Sara

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Hjort, KarinJansson, Janet K.Sjöling, Sara
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