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Profiling LPS Glycoforms of Non-typeable Haemophilus influenzae by Multiple-Stage Tandem Mass Spectrometry
Södertörns högskola, Institutionen för livsvetenskaper, Kemi.
2010 (Engelska)Ingår i: Functional glycomics: methods and protocols / [ed] Jianjun Li, New York: Humana Press, 2010, s. 79-92Kapitel i bok, del av antologi (Refereegranskat)
Abstract [en]

Non-typeable (acapsular) Haemophilus influenzae (NTHi) is a major cause of otitis media accounting for 25-30% of all cases of the disease. Lipopolysaccharide (LPS) is an essential and exposed component of the H. influenzae cell wall. A characteristic feature of H. influenzae LPS is the extensive inter-strain and intra-strain heterogeneity of glycoform structure which is key to the role of the molecule in both commensal and disease-causing behavior of the bacterium. However, to characterize LPS structure unambiguously is a major challenge due to the extreme heterogeneity of glycoforms that certain strains express. A powerful tool for obtaining sequence and branching information is multiple-stage tandem ESI-MS (ESI-MS(n)) performed on dephosphorylated and permethylated oligosaccharide material using an ESI-quadrupole ion trap mass spectrometer. In general, permethylation increases the MS response by several orders of magnitude and sequence information is readily obtained since methyl tagging allows the distinction between fragment ions generated by cleavage of a single glycosidic bond and inner fragments resulting from the rupture of two glycosidic linkages. Using this approach we are now able to identify all isomeric glycoforms in very heterogeneous LPS preparations.

Ort, förlag, år, upplaga, sidor
New York: Humana Press, 2010. s. 79-92
Serie
Methods in Molecular Biology, ISSN 1064-3745 ; 600
Nationell ämneskategori
Biokemi och molekylärbiologi
Identifikatorer
URN: urn:nbn:se:sh:diva-16213DOI: 10.1007/978-1-60761-454-8_6ISI: 000272399800006ISBN: 978-1-60761-453-1 (tryckt)OAI: oai:DiVA.org:sh-16213DiVA, id: diva2:529229
Tillgänglig från: 2012-05-29 Skapad: 2012-05-16 Senast uppdaterad: 2013-12-18Bibliografiskt granskad

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Schweda, Elke K H

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Totalt: 151 träffar
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