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Bacterial chitinase with phytopathogen control capacity from suppressive soil revealed by functional metagenomics
Södertörns högskola, Institutionen för naturvetenskap, miljö och teknik, Biologi. Uppsala University. (Sara Sjöling)
University of Insubria, Varese, Italy. (Sara Sjöling)
Södertörns högskola, Institutionen för naturvetenskap, miljö och teknik, Biologi. (Sara Sjöling)
University of Insubria, Varese, Italy.
Vise andre og tillknytning
2014 (engelsk)Inngår i: Applied Microbiology and Biotechnology, ISSN 0175-7598, E-ISSN 1432-0614, Vol. 98, nr 6, s. 2819-2828Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Plant disease caused by fungal pathogens results in vast crop damage globally. Microbial communities of soil that is suppressive to fungal crop disease provide a source for the identification of novel enzymes functioning as bioshields against plant pathogens. In this study, we targeted chitin-degrading enzymes of the uncultured bacterial community through a functional metagenomics approach, using a fosmid library of a suppressive soil metagenome. We identified a novel bacterial chitinase, Chi18H8, with antifungal activity against several important crop pathogens. Sequence analyses show that the chi18H8 gene encodes a 425-amino acid protein of 46 kDa with an N-terminal signal peptide, a catalytic domain with the conserved active site F175DGIDIDWE183, and a chitinase insertion domain. Chi18H8 was expressed (pGEX-6P-3 vector) in Escherichia coli and purified. Enzyme characterization shows that Chi18H8 has a prevalent chitobiosidase activity with a maximum activity at 35 °C at pH lower than 6, suggesting a role as exochitinase on native chitin. To our knowledge, Chi18H8 is the first chitinase isolated from a metagenome library obtained in pure form and which has the potential to be used as a candidate agent for controlling fungal crop diseases. Furthermore, Chi18H8 may also answer to the demand for novel chitin-degrading enzymes for a broad range of other industrial processes and medical purposes.

sted, utgiver, år, opplag, sider
2014. Vol. 98, nr 6, s. 2819-2828
Emneord [en]
metagenomic library, chitinase, terminal restriction fragment length polymorphism (T-RFLP), Streptomycetes, suppressive soil
HSV kategori
Forskningsprogram
Östersjö- och Östeuropaforskning; Miljövetenskapliga studier
Identifikatorer
URN: urn:nbn:se:sh:diva-20023DOI: 10.1007/s00253-013-5287-xISI: 000332108100041PubMedID: 24121932Scopus ID: 2-s2.0-84905997072Lokal ID: 47/42/2011OAI: oai:DiVA.org:sh-20023DiVA, id: diva2:659271
Prosjekter
Metaexplore - metagenomics for bioexploration
Forskningsfinansiär
EU, FP7, Seventh Framework Programme, KBBE-222625The Foundation for Baltic and East European Studies
Merknad

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Tilgjengelig fra: 2013-10-24 Laget: 2013-10-24 Sist oppdatert: 2019-03-04bibliografisk kontrollert

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Hjort et al 2013(848 kB)534 nedlastinger
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