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Degradation of GFP-labelled POM121, a non-invasive sensor of nuclear apoptosis, precedes clustering of nuclear pores and externalisation of phosphatidylserine
Södertörns högskola, Institutionen för kemi, biologi, geografi och miljövetenskap.
Södertörns högskola, Institutionen för kemi, biologi, geografi och miljövetenskap.
2004 (engelsk)Inngår i: Apoptosis (London), ISSN 1360-8185, E-ISSN 1573-675X, Vol. 9, nr 3, s. 363-368Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

The nuclear pore membrane protein POM121 is specifically degraded during apoptosis by a caspase-3-dependent process enabling early detection of apoptosis in living cells expressing POM121-GFP. Here we further investigated temporal aspects of apoptotic degradation of POM121-GFP. We demonstrate that decreased POM121-GFP fluorescence precedes annexin V-labelling of apoptotic cells. This indicates that degradation of the nuclear pore complex starts prior to redistribution of plasma membrane phosphatidylserine, which serves as a signal for phagocytotic elimination of apoptotic cells. Furthermore, a caspase-resistant GFP-labelled mutant of POM121 resisted degradation even in late apoptosis and was detected in clustered nuclear pores. Thus, it can be concluded that loss of POM121-GFP is a specific sensor of the activation of caspase-3-dependent proteolysis at the nuclear pores.

sted, utgiver, år, opplag, sider
2004. Vol. 9, nr 3, s. 363-368
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URN: urn:nbn:se:sh:diva-15480DOI: 10.1023/B:APPT.0000025813.75258.b5ISI: 000221102700012PubMedID: 15258468Scopus ID: 2-s2.0-3242805164OAI: oai:DiVA.org:sh-15480DiVA, id: diva2:504654
Tilgjengelig fra: 2012-02-21 Laget: 2012-02-20 Sist oppdatert: 2017-12-07bibliografisk kontrollert

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