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Developing electroporation as a method to obtain Stable Transformation in Drosophila melanogaster
Södertörns högskola, Institutionen för livsvetenskaper. (Drosophila lab.)
2008 (engelsk)Independent thesis Advanced level (degree of Master (Two Years)), 20 poäng / 30 hpOppgave
Abstract [en]

In this project I have tried to obtain stable transformants of Drosophila melanogaster flies using electroporation. I have completed approximately 200 tests using different DNA concentrations, voltages and cuvettes, including a novel Petri dish cuvette which I developed and manufactured myself. I also developed new and more efficient procedures of egg collection and egg dechorionation. Although I was not  successful in obtaining true stable transformants, control experiments indicate that electroporation of DNA into embryos could be accomplished under the conditions used. The lack of stable transformants was probably due to failure of the electroporated DNA to integrate into the host genome. The reasons for why the DNA did not integrate was not further investigated in this study.

sted, utgiver, år, opplag, sider
2008. , s. 28
Emneord [en]
Stable Transformation, electroporation, Drosophila melanogaster
HSV kategori
Identifikatorer
URN: urn:nbn:se:sh:diva-3245OAI: oai:DiVA.org:sh-3245DiVA, id: diva2:299663
Presentation
(engelsk)
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Life Earth Science
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Examiner
Tilgjengelig fra: 2010-02-23 Laget: 2010-02-23 Sist oppdatert: 2010-02-24bibliografisk kontrollert

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