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Detection of imipenem-resistant and metronidazole-resistant Bacteroides fragilis group strains in fecal samples
Södertörns högskola, Avdelning Naturvetenskap. Karolinska Institutet.
Södertörns högskola, Avdelning Naturvetenskap. Karolinska Institutet.
Karolinska Institutet.
Karolinska Institutet.
1999 (engelsk)Inngår i: Clinical Microbiology and Infection, ISSN 1198-743X, E-ISSN 1469-0691, Vol. 5, nr 12, s. 753-758Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Objective: To investigate the imipenem and metronidazole resistance profiles of Bacteroides fragilis group strains in fecal samples and to detect the resistance genes (ccrA and nim) coding for imipenem and metronidazole resistance in B. fragilis group strains. Methods: In total, 925 fecal samples, 729 from consecutive diarrhea patients and 196 from healthy controls, were collected at Huddinge University Hospital in 1997. A modified disk diffusion method was employed to screen for imipenem-resistant and metronidazole-resistant B. fragilis group strains. In strains considered resistant by the modified disk diffusion method, the minimum inhibitory concentrations (MICs) were further determined by the agar dilution method. PCR assays were used to detect the carbapenem-hydrolyzing metallo-P-lactamase gene (ccrA) and the 5-nitroimidazole resistance genes (nim) in pure cultures (purePCR), directly from fecal samples through direct broth enrichment (dirPCR) and by immunomagnetic separation (imsPCR). Results: Two imipenem-resistant B. fragilis strains, one of which was simultaneously resistant to metronidazole, and two B. fragilis group strains with MICs near the breakpoint for metronidazole resistance, were isolated from the fecal samples of diarrhea patients. The ccrA gene was identified in all the imipenem-resistant B. fragilis strains by purePCR, dirPCR and imsPCR. The nim genes were also detectable by these PCR assays. Conclusions: The incidences of imipenem-resistant and metronidazole-resistant B. fragilis group strains were low in the investigated diarrhea patients. Simultaneous resistance to imipenem and metronidazole is of great concern in clinical medicine, and the proposed PCR assays may be useful in epidemiologic studies of distribution of resistance genes in the fecal microflora.

sted, utgiver, år, opplag, sider
1999. Vol. 5, nr 12, s. 753-758
Emneord [en]
Bacteroides fragilis group, dirPCR, Imipenem, imsPCR, Metronidazole, purePCR, Resistance, agar, antibiotic agent, beta lactamase, antibiotic resistance, article, bacterial gene, bacterium detection, Bacteroides fragilis, cell separation, controlled study, diarrhea, diffusion, feces culture, human, major clinical study, minimum inhibitory concentration, nonhuman, polymerase chain reaction, priority journal, Bacteria (microorganisms)
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Identifikatorer
URN: urn:nbn:se:sh:diva-35668DOI: 10.1111/j.1469-0691.1999.tb00709.xScopus ID: 2-s2.0-0033381050OAI: oai:DiVA.org:sh-35668DiVA, id: diva2:1223380
Tilgjengelig fra: 2018-06-25 Laget: 2018-06-25 Sist oppdatert: 2018-06-25bibliografisk kontrollert

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