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Identification of metabolically active phenanthrene transforming bacteria in polluted Baltic Sea sediments
Södertörns högskola, Institutionen för livsvetenskaper. SLU.
SLU.
(engelsk)Manuskript (preprint) (Annet vitenskapelig)
HSV kategori
Forskningsprogram
Östersjö- och Östeuropaforskning
Identifikatorer
URN: urn:nbn:se:sh:diva-31260OAI: oai:DiVA.org:sh-31260DiVA, id: diva2:1051162
Merknad

Som manuskript i avhandling. As manuscript in dissertation.

Tilgjengelig fra: 2016-12-01 Laget: 2016-12-01 Sist oppdatert: 2016-12-01bibliografisk kontrollert
Inngår i avhandling
1. Microbial diversity in Baltic Sea sediments
Åpne denne publikasjonen i ny fane eller vindu >>Microbial diversity in Baltic Sea sediments
2007 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
Abstract [en]

This thesis focuses on microbial community structures and their functions in Baltic Sea sediments. First we investigated the distribution of archaea and bacteria in Baltic Sea sediments along a eutrophication gradient. Community profile analysis of 16S rRNA genes using terminal restriction length polymorphism (T-RFLP) indicated that archaeal and bacterial communities were spatially heterogeneous. By employing statistical ordination methods we observed that archaea and bacteria were structured and impacted differently by environmental parameters that were significantly linked to eutrophication. In a separate study, we analyzed bacterial communities at a different site in the Baltic Sea that was heavily contaminated with polyaromatic hydrocarbons (PAHs) and several other pollutants. Sediment samples were collected before and after remediation by dredging in two consecutive years. A polyphasic experimental approach was used to assess growing bacteria and degradation genes in the sediments. The bacterial communities were significantly different before and after dredging of the sediment. Several isolates collected from contaminated sediments showed an intrinsic capacity for degradation of phenanthrene (a PAH model compound). Quantititative real-time PCR was used to monitor the abundance of degradation genes in sediment microcosms spiked with phenanthrene. Although both xylE and phnAc genes increased in abundance in the microcosms, the isolates only carried phnAc genes. Isolates with closest 16S rRNA gene sequence matches to Exigobacterium oxidotolerans, a Pseudomonas sp. and a Gammaproteobacterium were identified by all approaches used as growing bacteria that are capable of phenanthrene degradation. These isolates were assigned species and strain designations as follows: Exiguobacterium oxidotolerans AE3, Pseudomonas fluorescens AE1 and Pseudomonas migulae AE2. We also identified and studied the distribution of actively growing bacteria along red-ox profiles in Baltic Sea sediments. Community structures were found to be significantly different at different red-ox depths. Also, according to multivariate statistical ordination analysis organic carbon, nitrogen, and red-ox potential were crucial parameters for structuring the bacterial communities on a vertical scale. Novel lineages of bacteria were obtained by sequencing 16S rRNA genes from different red-ox depths and sampling stations indicating that bacterial diversity in Baltic Sea sediments is largely unexplored.

sted, utgiver, år, opplag, sider
Uppsala: Sveriges Lantbruksuniversitet, 2007. s. 36
Serie
Acta Universitatis agriculturae Sueciae, ISSN 1652-6880 ; 2007:6
Emneord
Havsbottnen, Mikroorganismer
HSV kategori
Forskningsprogram
Östersjö- och Östeuropaforskning
Identifikatorer
urn:nbn:se:sh:diva-31261 (URN)91-576-7325-X (ISBN)
Veileder
Tilgjengelig fra: 2016-12-01 Laget: 2016-12-01 Sist oppdatert: 2016-12-01bibliografisk kontrollert

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