sh.sePublikasjoner
Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • harvard-anglia-ruskin-university
  • apa-old-doi-prefix.csl
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Toxigenic Vibrio cholerae identified in estuaries of Tanzania using PCR techniques
Amana Regional Referral Hospital, Dar es Salaam, Tanzania / University of Dar es Salaam, Dar es Salaam, Tanzania.
University of Dar es Salaam, Dar es Salaam, Tanzania.
University of Dar es Salaam, Dar es Salaam, Tanzania.
University of Dar es Salaam, Dar es Salaam, Tanzania.
Vise andre og tillknytning
2015 (engelsk)Inngår i: FEMS Microbiology Letters, ISSN 0378-1097, E-ISSN 1574-6968, Vol. 362, nr 5, artikkel-id fnv009Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

The current study assessed the occurrence of the Vibrio cholerae serogroups O1 and O139 in environmental samples along salinity gradients in three selected estuaries of Tanzania both through culture independent methods and by cultured bacteria. Occurrence of V. cholerae was determined by PCR targeting the V. cholerae outer membrane protein gene ompW. Furthermore, the presence of toxigenic strains and serogroups O1 and O139 was determined using multiplex PCR with specific primers targeting the cholera toxin gene subunit A, ctxA, and serotype specific primers, O1-rfb and O139-rfb, respectively. Results showed that V. cholerae occurred in approximately 10% (n = 185) of both the environmental samples and isolated bacteria. Eight of the bacteria isolates (n = 43) were confirmed as serogroup O1 while one belonged to serogroup O139, the first reported identification of this epidemic strain in East African coastal waters. All samples identified as serogroup O1 or O139 and a number of non-O1/O139 strains were ctxA positive. This study provides in situ evidence of the presence of pathogenic V. cholerae O1 and O139 and a number of V. cholerae non-O1/O139 that carry the cholera toxin gene in estuaries along the coast of Tanzania.

sted, utgiver, år, opplag, sider
2015. Vol. 362, nr 5, artikkel-id fnv009
HSV kategori
Forskningsprogram
Miljövetenskapliga studier
Identifikatorer
URN: urn:nbn:se:sh:diva-26569DOI: 10.1093/femsle/fnv009ISI: 000356885500010PubMedID: 25743072Scopus ID: 2-s2.0-84952360466OAI: oai:DiVA.org:sh-26569DiVA, id: diva2:794919
Tilgjengelig fra: 2015-03-13 Laget: 2015-03-12 Sist oppdatert: 2018-04-05bibliografisk kontrollert

Open Access i DiVA

Fulltekst mangler i DiVA

Andre lenker

Forlagets fulltekstPubMedScopus

Personposter BETA

Sjöling, Sara

Søk i DiVA

Av forfatter/redaktør
Sjöling, Sara
Av organisasjonen
I samme tidsskrift
FEMS Microbiology Letters

Søk utenfor DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric

doi
pubmed
urn-nbn
Totalt: 207 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • harvard-anglia-ruskin-university
  • apa-old-doi-prefix.csl
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf