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Lee, Hang-mao
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Wiren, M., Silverstein, R. A., Sinha, I., Walfridsson, J., Lee, H.-m., Laurenson, P., . . . Ekwall, K. (2005). Genomewide analysis of nucleosome density histone acetylation and HDAC function in fission yeast. EMBO Journal, 24(16), 2906-2918
Open this publication in new window or tab >>Genomewide analysis of nucleosome density histone acetylation and HDAC function in fission yeast
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2005 (English)In: EMBO Journal, ISSN 0261-4189, E-ISSN 1460-2075, Vol. 24, no 16, p. 2906-2918Article in journal (Refereed) Published
Abstract [en]

We have conducted a genomewide investigation into the enzymatic specificity, expression profiles, and binding locations of four histone deacetylases (HDACs), representing the three different phylogenetic classes in fission yeast ( Schizosaccharomyces pombe). By directly comparing nucleosome density, histone acetylation patterns and HDAC binding in both intergenic and coding regions with gene expression profiles, we found that Sir2 ( class III) and Hos2 ( class I) have a role in preventing histone loss; Clr6 ( class I) is the principal enzyme in promoter-localized repression. Hos2 has an unexpected role in promoting high expression of growth-related genes by deacetylating H4K16Ac in their open reading frames. Clr3 ( class II) acts cooperatively with Sir2 throughout the genome, including the silent regions: rDNA, centromeres, mat2/3 and telomeres. The most significant acetylation sites are H3K14Ac for Clr3 and H3K9Ac for Sir2 at their genomic targets. Clr3 also affects subtelomeric regions which contain clustered stress- and meiosis-induced genes. Thus, this combined genomic approach has uncovered different roles for fission yeast HDACs at the silent regions in repression and activation of gene expression.

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Biochemistry and Molecular Biology
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urn:nbn:se:sh:diva-14447 (URN)10.1038/sj.emboj.7600758 (DOI)000231789300007 ()16079916 (PubMedID)2-s2.0-72949109535 (Scopus ID)
Available from: 2012-01-19 Created: 2011-12-23 Last updated: 2017-07-19Bibliographically approved
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