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Söll, Iris
Publications (5 of 5) Show all publications
Bräutigam, L., Hillmer, J. M., Söll, I. & Hauptmann, G. (2010). Localized Expression of Urocortin Genes in the Developing Zebrafish rain. Journal of Comparative Neurology, 518(15), 2978-2995
Open this publication in new window or tab >>Localized Expression of Urocortin Genes in the Developing Zebrafish rain
2010 (English)In: Journal of Comparative Neurology, ISSN 0021-9967, E-ISSN 1096-9861, Vol. 518, no 15, p. 2978-2995Article in journal (Refereed) Published
Abstract [en]

The corticotropin-releasing hormone (CRH) family consists of four aralogous genes, CRH and urocortins (UCNs) 1, 2, and 3. In a previous tudy, we analyzed CRH in the teleost model organism zebrafish and its ranscript distribution in the embryonic brain. Here, we describe ull-length cDNAs encoding urotensin 1 (UTS1), the teleost UCN1 rtholog, and UCN3 of zebrafish. Major expression sites of uts1 in adult ebrafish are the caudal neurosecretory system and brain. By using T-PCR analysis, we show that uts1 mRNA is also present in ovary, aternally contributed to the embryo, and expressed throughout embryonic evelopment. Expression of ucn3 mRNA was detected in a range of adult issues and during developmental stages from 24 hours post fertilization nward. Analysis of spatial transcript distributions by whole-mount in itu hybridization revealed limited forebrain expression of uts1 and cn3 during early development. Small numbers of uts1-synthesizing eurons were found in subpallium, hypothalamus, and posterior iencephalon, whereas ucn3-positive cells were restricted to elencephalon and retina. The brainstem was the main site of uts1 and cn3 synthesis in the embryonic brain. uts1 Expression was confined to he midbrain tegmentum; distinct hindbrain cell groups, including locus oeruleus and Mauthner neurons; and the spinal cord. ucn3 Expression was ocalized to the optic tectum, serotonergic raphe, and distinct hombomeric cell clusters. The prominent expression of uts1 and ucn3 in rainstem is consistent with proposed roles of CRH-related peptides in tress-induced modulation of locomotor activity through monoaminergic rainstem neuromodulatory systems. J. Comp. Neurol. 518:2978-2995, 2010.

National Category
Neurosciences Zoology
Identifiers
urn:nbn:se:sh:diva-13705 (URN)10.1002/cne.22375 (DOI)000279116500005 ()2-s2.0-77954576452 (Scopus ID)
Available from: 2011-12-06 Created: 2011-12-06 Last updated: 2018-01-12Bibliographically approved
Jensen, L. D., Cao, R., Hedlund, E.-M., Söll, I., Lundberg, J. O., Hauptmann, G., . . . Cao, Y. (2009). Nitric oxide permits hypoxia-induced lymphatic perfusion by controlling arterial-lymphatic conduits in zebrafish and glass catfish. Proceedings of the National Academy of Sciences of the United States of America, 106(43), 18408-18413
Open this publication in new window or tab >>Nitric oxide permits hypoxia-induced lymphatic perfusion by controlling arterial-lymphatic conduits in zebrafish and glass catfish
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2009 (English)In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 106, no 43, p. 18408-18413Article in journal (Refereed) Published
Abstract [en]

The blood and lymphatic vasculatures are structurally and functionally coupled in controlling tissue perfusion, extracellular interstitial fluids, and immune surveillance. Little is known, however, about the molecular mechanisms that underlie the regulation of bloodlymphatic vessel connections and lymphatic perfusion. Here we show in the adult zebrafish and glass catfish (Kryptopterus bicirrhis) that blood-lymphatic conduits directly connect arterial vessels to the lymphatic system. Under hypoxic conditions, arterial-lymphatic conduits (ALCs) became highly dilated and linearized by NO-induced vascular relaxation, which led to blood perfusion into the lymphatic system. NO blockage almost completely abrogated hypoxia-induced ALC relaxation and lymphatic perfusion. These findings uncover mechanisms underlying hypoxia-induced oxygen compensation by perfusion of existing lymphatics in fish. Our results might also imply that the hypoxia-induced NO pathway contributes to development of progression of pathologies, including promotion of lymphatic metastasis by modulating arterial-lymphatic conduits, in the mammalian system.

National Category
Biological Sciences
Identifiers
urn:nbn:se:sh:diva-13879 (URN)10.1073/pnas.0907608106 (DOI)000271222500066 ()19822749 (PubMedID)2-s2.0-70849085474 (Scopus ID)
Available from: 2011-12-14 Created: 2011-12-14 Last updated: 2017-07-18Bibliographically approved
Lauter, G., Söll, I. & Hauptmann, G. (2009). PACAP in zebrafish neurodevelopment. Paper presented at 16th Annual Conference of the International-Society-of-Development-Biologists, SEP 06-10, 2009, Edinburgh, SCOTLAND. Mechanisms of Development, 126, S235-S235
Open this publication in new window or tab >>PACAP in zebrafish neurodevelopment
2009 (English)In: Mechanisms of Development, ISSN 0925-4773, E-ISSN 1872-6356, Vol. 126, p. S235-S235Article in journal, Meeting abstract (Refereed) Published
National Category
Biological Sciences
Identifiers
urn:nbn:se:sh:diva-35659 (URN)10.1016/j.mod.2009.06.606 (DOI)000270034900687 ()
Conference
16th Annual Conference of the International-Society-of-Development-Biologists, SEP 06-10, 2009, Edinburgh, SCOTLAND
Available from: 2018-06-25 Created: 2018-06-25 Last updated: 2018-06-25Bibliographically approved
Gabriel, J. P., Mahmood, R., Kyriakatos, A., Söll, I., Hauptmann, G., Calabrese, R. L. & El Manira, A. (2009). Serotonergic Modulation of Locomotion in Zebrafish-Endogenous Release and Synaptic Mechanisms. Journal of Neuroscience, 29(33), 10387-10395
Open this publication in new window or tab >>Serotonergic Modulation of Locomotion in Zebrafish-Endogenous Release and Synaptic Mechanisms
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2009 (English)In: Journal of Neuroscience, ISSN 0270-6474, E-ISSN 1529-2401, Vol. 29, no 33, p. 10387-10395Article in journal (Refereed) Published
Abstract [en]

Serotonin (5-HT) plays an important role in shaping the activity of the spinal networks underlying locomotion in many vertebrate preparations. At larval stages in zebrafish, 5-HT does not change the frequency of spontaneous swimming; and it only decreases the quiescent period between consecutive swimming episodes. However, it is not known whether 5-HT exerts similar actions on the locomotor network at later developmental stages. For this, the effect of 5-HT on the fictive locomotor pattern of juvenile and adult zebrafish was analyzed. Bath-application of 5-HT (1-20 mu M) reduced the frequency of the NMDA-induced locomotor rhythm. Blocking removal from the synaptic cleft with the reuptake inhibitor citalopram had similar effects, suggesting that endogenous serotonin is modulating the locomotor pattern. One target for this modulation was the mid-cycle inhibition during locomotion because the IPSPs recorded in spinal neurons during the hyperpolarized phase were increased both in amplitude and occurrence by 5-HT. Similar results were obtained for IPSCs recorded in spinal neurons clamped at the reversal potential of excitatory currents (0 mV). 5-HT also slows down the rising phase of the excitatory drive recorded in spinal cord neurons when glycinergic inhibition is blocked. These results suggest that the decrease in the locomotor burst frequency induced by 5-HT is mediated by a potentiation of mid-cycle inhibition combined with a delayed onset of the subsequent depolarization.

National Category
Neurosciences
Identifiers
urn:nbn:se:sh:diva-13888 (URN)10.1523/JNEUROSCI.1978-09.2009 (DOI)000269087300022 ()19692613 (PubMedID)2-s2.0-69049085614 (Scopus ID)
Available from: 2011-12-14 Created: 2011-12-14 Last updated: 2018-01-12Bibliographically approved
Cao, R., Jensen, L. D., Söll, I., Hauptmann, G. & Cao, Y. (2008). Hypoxia-Induced Retinal Angiogenesis in Zebrafish as a Model to Study Retinopathy. PLoS ONE, 3(7), e2748
Open this publication in new window or tab >>Hypoxia-Induced Retinal Angiogenesis in Zebrafish as a Model to Study Retinopathy
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2008 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 3, no 7, p. e2748-Article in journal (Refereed) Published
Abstract [en]

Mechanistic understanding and defining novel therapeutic targets of diabetic retinopathy and age-related macular degeneration (AMD) have been hampered by a lack of appropriate adult animal models. Here we describe a simple and highly reproducible adult fli-EGFP transgenic zebrafish model to study retinal angiogenesis. The retinal vasculature in the adult zebrafish is highly organized and hypoxia-induced neovascularization occurs in a predictable area of capillary plexuses. New retinal vessels and vascular sprouts can be accurately measured and quantified. Orally active anti-VEGF agents including sunitinib and ZM323881 effectively block hypoxia-induced retinal neovascularization. Intriguingly, blockage of the Notch signaling pathway by the inhibitor DAPT under hypoxia, results in a high density of arterial sprouting in all optical arteries. The Notch suppression-induced arterial sprouting is dependent on tissue hypoxia. However, in the presence of DAPT substantial endothelial tip cell formation was detected only in optic capillary plexuses under normoxia. These findings suggest that hypoxia shifts the vascular targets of Notch inhibitors. Our findings for the first time show a clinically relevant retinal angiogenesis model in adult zebrafish, which might serve as a platform for studying mechanisms of retinal angiogenesis, for defining novel therapeutic targets, and for screening of novel antiangiogenic drugs.

National Category
Biological Sciences
Identifiers
urn:nbn:se:sh:diva-14141 (URN)10.1371/journal.pone.0002748 (DOI)000264302900019 ()2-s2.0-50249086022 (Scopus ID)
Available from: 2011-12-18 Created: 2011-12-16 Last updated: 2017-12-08Bibliographically approved
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