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Hallgren, Stefan
Publications (10 of 16) Show all publications
Volkova, K., Caspillo, N. R., Porseryd, T., Hallgren, S., Dinnétz, P. & Porsch-Hällström, I. (2015). Developmental exposure of zebrafish (Danio rerio) to 17α-Ethinylestradiol affects non-reproductive behavior and fertility as adults, and increases anxiety in unexposed progeny. Hormones and Behavior, 73, 30-38
Open this publication in new window or tab >>Developmental exposure of zebrafish (Danio rerio) to 17α-Ethinylestradiol affects non-reproductive behavior and fertility as adults, and increases anxiety in unexposed progeny
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2015 (English)In: Hormones and Behavior, ISSN 0018-506X, E-ISSN 1095-6867, Vol. 73, p. 30-38Article in journal (Refereed) Published
Abstract [en]

Exposure to estrogenic endocrine disruptors (EDCs) during of development affects fertility, reproductive and non-reproductive behavior in mammals and fish. These effects can also be transferred to coming generations. In fish, the effects of developmental EDC exposure on non-reproductive behavior is less well studied. Here, we analyze the effects of 17α-Ethinylestradiol (EE2) on anxiety, shoaling behavior and fertility in zebrafish after developmental treatment and remediation in clean water until adulthood. Zebrafish embryos were exposed from day 1 to day 80 post fertilization to actual concentrations of 1.2 and 1.6ng/L EE2. After remediation for 82days non-reproductive behavior and fertilization success were analyzed in both sexes. Males and females from the 1.2ng/L group, as well as control males and females, were bred, and behavior of the untreated F1 offspring was tested as adults. Developmental treatment with 1.2 and 1.6ng/L EE2 significantly increased anxiety in the Novel Tank test and increased shoaling intensity in both sexes. Fertilization success was significantly reduced by EE2 in both sexes when mated with untreated fish of opposite sex. Progeny of fish treated with 1.2ng/L EE2 showed increased anxiety in the Novel tank test and increased light avoidance in the Scototaxis test compared to control offspring. In conclusion, developmental exposure of zebrafish to low doses of EE2 resulted in persistent changes in behavior and fertility. The behavior of unexposed progeny were affected by their parents' exposure, which might suggest transgenerational effects.

Keywords
17α-Ethinylestradiol; Anxiety; Developmental exposure; Endocrine disruptors; F1 effects; Fertility; Neuroendocrinology; Social behavior; Stress behavior; Zebrafish
National Category
Biological Sciences Environmental Sciences
Research subject
Environmental Studies; Baltic and East European studies
Identifiers
urn:nbn:se:sh:diva-27795 (URN)10.1016/j.yhbeh.2015.05.014 (DOI)000360251800005 ()26072466 (PubMedID)2-s2.0-84934983120 (Scopus ID)1556/42/2011 (Local ID)1556/42/2011 (Archive number)1556/42/2011 (OAI)
Projects
Endocrine disruption in fish
Funder
The Foundation for Baltic and East European Studies, 1742/42/2008The Foundation for Baltic and East European Studies, 1556/42/2011
Available from: 2015-06-18 Created: 2015-06-18 Last updated: 2019-04-18Bibliographically approved
Volkova, K., Reyhanian Caspillo, N., Porseryd, T., Hallgren, S., Dinnetz, P., Olsén, H. & Porsch Hällström, I. (2015). Transgenerational effects of 17α-Ethinyl Estradiol on anxiety behavior in the guppy, Poecilia reticulata. General and Comparative Endocrinology, 223, 66-72
Open this publication in new window or tab >>Transgenerational effects of 17α-Ethinyl Estradiol on anxiety behavior in the guppy, Poecilia reticulata
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2015 (English)In: General and Comparative Endocrinology, ISSN 0016-6480, E-ISSN 1095-6840, Vol. 223, p. 66-72Article in journal (Refereed) Published
National Category
Zoology Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:sh:diva-28427 (URN)10.1016/j.ygcen.2015.09.027 (DOI)000366438300008 ()26431611 (PubMedID)2-s2.0-84944226825 (Scopus ID)
Note

Som manuskript i avhandling. As manuscript in dissertation.

Available from: 2015-09-30 Created: 2015-10-01 Last updated: 2019-04-18Bibliographically approved
Olsén, K. H., Ask, K., Olsén, H., Porsch-Hällström, I. & Hallgren, S. (2014). Effects of the SSRI citalopram on behaviours connected to stress and reproduction in Endler guppy, Poecilia wingei . Aquatic Toxicology, 148, 113-121
Open this publication in new window or tab >>Effects of the SSRI citalopram on behaviours connected to stress and reproduction in Endler guppy, Poecilia wingei
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2014 (English)In: Aquatic Toxicology, ISSN 0166-445X, E-ISSN 1879-1514, Vol. 148, p. 113-121Article in journal (Refereed) Published
Abstract [en]

Psychoactive drugs, such as selective serotonin reuptake inhibitors (SSRI) have been identified in high levels in effluents from Swedish sewage treatment plants (STP) at concentrations high enough to give pharmacological effects in fish. In humans SSRIs are used in the treatment of depression and they have anxiolytic effects. In the present study we exposed Endler guppy (Poecilia wingei) of both sexes to citalopram that showed the highest concentrations of SSRIs in STP effluents and studied reproductive and non-reproductive behaviour. Male courting behaviours were not affected compared to control fish after 14-28 days exposure to 1μgL-1. In two experiments exposing both sexes to 0.2, 2.3 or 15μgL-1 for 21 days, fish exposed to the two highest doses showed anxiolytic effects when placed in a novel environment (novel tank diving test, NT). Males were only affected by exposure to 15μgL-1. They had significantly longer latency to explore the upper half of the aquarium, more visits and longer time spent in the upper half, and showed less bottom freezing behaviour, all markers of anxiolytic behaviour. In females exposure to 2.3 or 15μgL-1 significantly increased freezing behaviour, while no effects on other behaviour variables were observed. No effects on shoaling behaviour could be discerned. These results show that citalopram have anxiolytic effects on guppy fish and thus affect ecologically relevant behaviours of importance to survival of fish.

Keywords
SSRI, Citalopram, behaviour, guppy
National Category
Biological Sciences
Research subject
Environmental Studies
Identifiers
urn:nbn:se:sh:diva-20977 (URN)10.1016/j.aquatox.2013.12.032 (DOI)000333496200013 ()24473162 (PubMedID)2-s2.0-84892895423 (Scopus ID)
Projects
Läkemedel i miljön
Funder
The Foundation for Baltic and East European Studies
Note

Reprint in: Aquatic Toxicology 151 (2014) 97-104. DOI: 10.1016/j.aquatox.2014.02.011

Available from: 2014-01-02 Created: 2014-01-02 Last updated: 2018-05-22Bibliographically approved
Reyhanian Caspillo, N., Volkova, K., Hallgren, S., Olsson, P.-E. & Porsch-Hällström, I. (2014). Short-term treatment of adult male zebrafish (Danio Rerio) with 17α-ethinyl estradiol affects the transcription of genes involved in development and male sex differentiation.. Comparative Biochemistry and Physiology - Part C: Toxicology & Pharmacology, 164, 35-42
Open this publication in new window or tab >>Short-term treatment of adult male zebrafish (Danio Rerio) with 17α-ethinyl estradiol affects the transcription of genes involved in development and male sex differentiation.
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2014 (English)In: Comparative Biochemistry and Physiology - Part C: Toxicology & Pharmacology, ISSN 1532-0456, E-ISSN 1878-1659, Vol. 164, p. 35-42Article in journal (Refereed) Published
Abstract [en]

The synthetic estrogen 17α-ethinyl estradiol (EE2) disturbs reproduction and causes gonadal malformation in fish. Effects on the transcription of genes involved in gonad development and function that could serve as sensitive biomarkers of reproductive effects in the field is, however, not well known. We have studied mRNA expression in testes and liver of adult zebrafish (Danio rerio) males treated with 0, 5 or 25ng/L EE2for 14days. qPCR analysis showed that the mRNA expression of four genes linked to zebrafish male sex determination and differentiation, Anti-Mullerian Hormone, Double sex and mab-related protein, Sry-related HMG box-9a and Nuclear receptor subfamily 5 group number 1b were significantly decreased by 25ng/L, but not 5ng/L EE2 compared with the levels in untreated fish. The decreased transcription was correlated with a previously shown spawning failure in these males (Reyhanian et al., 2011. Aquat Toxicol 105, 41-48), suggesting that decreased mRNA expression of genes regulating male sexual function could be involved in the functional sterility. The mRNA level of Cytochrome P-45019a, involved in female reproductive development, was unaffected by hormone treatment. The transcription of the female-specific Vitellogenin was significantly induced in testes. While testicular Androgen Receptor and the Estrogen Receptor-alpha mRNA levels were unchanged, Estrogen receptor-beta was significantly decreased by 25ng/L EE2. Hepatic Estrogen Receptor-alpha mRNA was significantly increased by both exposure concentrations, while Estrogen Receptor-beta transcription was unaltered. The decreased transcription of male-predominant genes supports a demasculinization of testes by EE2 and might reflect reproductive disturbances in the environment.

Keywords
landscape scars, industrial heritage, waste, nuclear power, mining, Malmberget, Ignalina, Sweden, Lithuania
National Category
Biological Sciences
Identifiers
urn:nbn:se:sh:diva-23362 (URN)10.1016/j.cbpc.2014.04.003 (DOI)000337769100005 ()24747828 (PubMedID)2-s2.0-84899872779 (Scopus ID)
Funder
The Foundation for Baltic and East European Studies
Available from: 2014-04-25 Created: 2014-04-25 Last updated: 2017-12-05Bibliographically approved
Volkova, K., Reyhanian, N., Kot-Wasik, A., Olsén, H., Porsch-Hällström, I. & Hallgren, S. (2012). Brain circuit imprints of developmental 17α-ethinylestradiol exposure in guppies (Poecilia reticulata): Persistent effects on anxiety but not on reproductive behaviour. General and Comparative Endocrinology, 178(2), 282-290
Open this publication in new window or tab >>Brain circuit imprints of developmental 17α-ethinylestradiol exposure in guppies (Poecilia reticulata): Persistent effects on anxiety but not on reproductive behaviour
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2012 (English)In: General and Comparative Endocrinology, ISSN 0016-6480, E-ISSN 1095-6840, Vol. 178, no 2, p. 282-290Article in journal (Refereed) Published
Abstract [en]

The effects of endocrine disruptors may vary with the timing of exposure. The physiological implications of adult exposure are present during and shortly after exposure while embryonic exposure can imprint changes manifested in adulthood. In this study, guppy (Poecilia reticulata) embryos were exposed to 2 ng/L and 20 ng/L of 17α-ethinylestradiol during development via the mother and reared in clean water from gestation until 6 months of age. As adults, fish exposed to 20ng/L during development showed significantly altered behaviour in the Novel Tank test, where anxiety is determined as the tendency to remain at the bottom upon introduction into an unfamiliar tank. 17α-ethinylestradiol treatment increased the latency time before swimming to the upper half of the tank and decreased the number of transitions to the upper half. In control females the basal stress behaviour responses were significantly higher than in males, as indicated by longer latency period and fewer and shorter visits to the upper half, supporting the importance of gonadal hormones for the behaviour. The anxiety increased, however, with treatment in both sexes, suggesting that the observed response is not entirely due to feminization of the males. Shoaling behaviour, analyzed as tendency to leave a shoal of littermates, was neither sex-differentiated nor changed by treatment. Also male reproductive behaviour, brain aromatase activity and testes histology, previously shown to respond to oestrogen exposure in adult guppy, were unaffected by the developmental treatment. This suggests that the stress system in the guppy is very sensitive to 17α-ethinylestradiol, which possibly causes an early organisational imprint on the brain circuit that regulates stress reactions.

Keywords
Endocrine disruption, Xenoestrogens, Sexual dimorphism, Anxiety, Stress behaviour, gonad histology, embryonic exposure
National Category
Biochemistry and Molecular Biology Environmental Sciences
Research subject
Environmental Studies
Identifiers
urn:nbn:se:sh:diva-16353 (URN)10.1016/j.ygcen.2012.05.010 (DOI)000307694100013 ()2-s2.0-84863217808 (Scopus ID)
Funder
The Foundation for Baltic and East European Studies
Available from: 2012-06-07 Created: 2012-06-07 Last updated: 2017-12-07Bibliographically approved
Archer, A., Srinivas Kitambi, S., Hallgren, S., Pedrelli, M., Olsén, H., Mode, A. & Gustafsson, J.-Å. (2012). The Liver X-Receptor (Lxr) Governs Lipid Homeostasis in Zebrafish during Development. Open journal of endocrine and metabolic diseases, 2(4), 74-81
Open this publication in new window or tab >>The Liver X-Receptor (Lxr) Governs Lipid Homeostasis in Zebrafish during Development
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2012 (English)In: Open journal of endocrine and metabolic diseases, ISSN 2165-7424, Vol. 2, no 4, p. 74-81Article in journal (Refereed) Published
Abstract [en]

The liver-X-receptors (LXRs) act as cholesterol sensors and participate in the regulation of lipid and cholesterol metabolism. The objective of this study was to determine the role of LXR during development using the zebrafish model. By in situ hybridization we showed distinct expression of lxr in the brain and the retina in the developing and adult zebrafish. Lxr ligand activation affected the expression of genes involved in lipid metabolism in zebrafish adult brain and eye as well as in zebrafish embryos. Morpholino knock down of lxr resulted in an overall impaired lipid deposition as determined by oil red O staining particularly in the head and around the eyes, and to significantly elevated levels of both total and free cholesterol in the yolk of lxr morphant embryos. The expression of genes involved in lipid and cholesterol metabolism was also changed in the lxr morphants. Furthermore, alcian blue staining revealed malformation of the pharyngeal skeleton in the lxr morphant. Our data show that Lxr is an important component of the regulatory network governing the lipid homeostasis during zebrafish development, which in turn may support a role of Lxr for normal development of the central nervous sytem, including the retina.

Place, publisher, year, edition, pages
Scientific Research Publishing, 2012
Keywords
Liver X receptor, Zebrafish, Morpholino, Lipid
National Category
Biological Sciences
Research subject
Other research area
Identifiers
urn:nbn:se:sh:diva-16973 (URN)10.4236/ojemd.2012.24012 (DOI)
Available from: 2012-08-27 Created: 2012-08-27 Last updated: 2015-01-15Bibliographically approved
Reyhanian, N., Volkova, K., Hallgren, S., Bollner, T., Olsson, P.-E., Olsén, K. H. & Porsch Hällström, I. (2011). 17α-Ethinyl estradiol affects anxiety and shoaling behavior in adult male zebra fish (Danio rerio). Aquatic Toxicology, 105, 41-48
Open this publication in new window or tab >>17α-Ethinyl estradiol affects anxiety and shoaling behavior in adult male zebra fish (Danio rerio)
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2011 (English)In: Aquatic Toxicology, ISSN 0166-445X, E-ISSN 1879-1514, Vol. 105, p. 41-48Article in journal (Refereed) Published
Keywords
Endocrine disruption, Behavior, Zebra fish, Anxiety, Shoaling, Ethinyl estradiol
National Category
Biological Sciences
Identifiers
urn:nbn:se:sh:diva-9406 (URN)10.1016/j.aquatox.2011.05.009 (DOI)000294317500005 ()2-s2.0-79960336976 (Scopus ID)
Available from: 2011-06-22 Created: 2011-06-22 Last updated: 2017-12-11Bibliographically approved
Hallgren, S., Volkova, K., Reyhanian, N., Olsén, K. H. & Porsch Hällström, I. (2011). Anxiogenic behaviour induced by 17α-ethynylestradiol in male guppies (Poecilia reticulata). Fish Physiology & Biochemistry, 37(4), 911-918
Open this publication in new window or tab >>Anxiogenic behaviour induced by 17α-ethynylestradiol in male guppies (Poecilia reticulata)
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2011 (English)In: Fish Physiology & Biochemistry, ISSN 0920-1742, E-ISSN 1573-5168, Vol. 37, no 4, p. 911-918Article in journal (Refereed) Published
Abstract [en]

Behaviour studies are used in toxicology research as they are excellent tools to measure physiological end-points caused by exogenous chemicals. In mammals both reproductive and non-reproductive behaviours have been used for a long period of time, whereas in teleost fishes non-reproductive behaviours have received little attention compared to reproductive behaviours. Recent advances in measuring stress related behaviours in zebrafish have provided additional tools to understand behaviour toxicology in fish. One species with well documented reproductive behaviour disturbed by different toxicants is the guppy, which is better suited than zebrafish for reproductive behaviour studies and therefore might be a better model organism for comparative behaviour studies in fish toxicology. Here we report new applications for non-reproductive behaviours in guppy and test these behaviours on males treated with the endocrine disruptor 17α-ethynylestradiol at environmentally relevant concentrations. 17α-ethynylestradiol increased freezing and bottom-dwelling when fish were placed in a non-familiar aquarium, but did not significantly affect shoaling behaviour. These results are similar to the anxiogenic behaviours seen in rats treated perinatally with 17α-ethynylestradiol and add more concern to the impacts of endocrine disruptors on aquatic wildlife.

National Category
Biological Sciences
Identifiers
urn:nbn:se:sh:diva-8616 (URN)10.1007/s10695-011-9488-x (DOI)000296646800018 ()21537944 (PubMedID)2-s2.0-80355132249 (Scopus ID)
Available from: 2011-05-17 Created: 2011-05-17 Last updated: 2017-12-11Bibliographically approved
Hallgren, S. & Olsén, K. H. (2010). Effects on Guppy Brain Aromatase Activity Following Short-Term Steroid and 4-Nonylphenol Exposures. Environmental Toxicology, 25(3), 261-271
Open this publication in new window or tab >>Effects on Guppy Brain Aromatase Activity Following Short-Term Steroid and 4-Nonylphenol Exposures
2010 (English)In: Environmental Toxicology, ISSN 1520-4081, E-ISSN 1522-7278, Vol. 25, no 3, p. 261-271Article in journal (Refereed) Published
Abstract [en]

Brain estrogen production, performed by the enzyme aromatase, can be disrupted/affected in teleost fish exposed to endocrine disruptors found in polluted aquatic environments. The guppy (Poecilia reticulata) was previously studied and confirmed to suffer negative effects on reproductive behaviors following inhibition of the brain aromatase reaction. Here adult guppies (Poecilia reticulata) of both genders were subjected to known endocrine disruptors: the androgen androstenedione (A), the synthetic estrogen 17 alpha-ethinylestradiol (EE2), and the estrogenic surfactant 4-nonylphenol (NP), at high (50 mu g/L) and at environmentally relevant concentrations (10 ng/L EE2, 5 mu g/L NP, and 0.7 mu g/L A) for 2 weeks followed by measurements of brain aromatase activity (bAA). In the adult males, bAA was stimulated by A and EE2 at 50 mu g/L. Female activity was also stimulated by the higher estrogenic treatment. At environmentally relevant concentrations only the EE2 treatment affected bAA, and only in males. The alkylphenolic substance NP produced no effect in either of the experiments, not on males nor females. The results indicate that short-term steroid treatments have stimulatory effects on guppy brain aromatase even at concentrations that can be found in the environment. We thus suggest bAA of adult guppies to be a suitable bioindicator of endocrine disruptors.

National Category
Environmental Sciences Biological Sciences
Identifiers
urn:nbn:se:sh:diva-5833 (URN)10.1002/tox.20494 (DOI)000277689000007 ()19489062 (PubMedID)2-s2.0-77954358974 (Scopus ID)
Available from: 2011-02-01 Created: 2011-01-31 Last updated: 2017-12-11Bibliographically approved
Hallgren, S. (2009). Brain aromatase in the guppy, Poecilia reticulata: distribution, control and role in behaviour. (Doctoral dissertation). Stockholm: Zoologiska institutionen, Stockholms universitet
Open this publication in new window or tab >>Brain aromatase in the guppy, Poecilia reticulata: distribution, control and role in behaviour
2009 (English)Doctoral thesis, comprehensive summary (Other academic)
Place, publisher, year, edition, pages
Stockholm: Zoologiska institutionen, Stockholms universitet, 2009. p. 41
Series
Södertörn Doctoral Dissertations, ISSN 1652-7399 ; 36
National Category
Biological Sciences
Identifiers
urn:nbn:se:sh:diva-8387 (URN)978-91-7155-870-1 (ISBN)978-91-86069-03-2 (ISBN)
Available from: 2011-05-05 Created: 2011-05-05 Last updated: 2015-06-14Bibliographically approved
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