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Bollner, Tomas
Publications (10 of 10) Show all publications
Mutvei, A., Bollner, T. & Mattsson, J.-E. (2015). Evolution, Teaching and Assessment of Students in Pre-Service Primary School Teacher Education. In: Conference proceedings. New perspectives in science education, 4th ed.: . Paper presented at New perspectives in science education, 4th ed, Florence, March 20-21, 2015.. Libreriauniversitaria.it
Open this publication in new window or tab >>Evolution, Teaching and Assessment of Students in Pre-Service Primary School Teacher Education
2015 (English)In: Conference proceedings. New perspectives in science education, 4th ed., Libreriauniversitaria.it , 2015, p. -3Conference paper, Published paper (Refereed)
Abstract [en]

This project aims at assessment of learning outcome of teaching evolutionary theory to pre-service primary school teacher students. The quality of the students understanding and use of evolutionary theory after their participation in seminars and lectures was made by analysing written reflections of the students. The texts were primarily written by students to show that the knowledge requirements of the course were accomplished but they were here used to analyse their ability to communicate the content but also to evaluate the depth of their understanding of evolutionary theory. For this assessment two different methods were used aiming at the two different targets. One was focused on the students’ use of evolutionary terminology and to what extent they used the language of an evolutionist. Here, we also analysed whether the students gave accurate explanations or descriptions without using the traditional evolutionary terminology. Thus, mainly the students´ ability in their own writing to present evolutionary reflections in an everyday language was here investigated. This ability may be useful when teaching young students without using a theoretical framework. For the assessment of quality of the students’ own understanding of the theory Doll’s criteria, the four R´s, richness, recursion, relations, and rigor, were used. Richness refers to the depth, the layers of meaning in their texts. Recursion is here referring to the students’ use of making thoughts loop back on themselves and earlier experiences. The use of relations are important when developing thinking in different areas. Here, both the students’ references to relations to other persons as well as to objects, theories, places etc. were recorded. Rigor means in this context to purposely look for different alternatives, relations, connections, new combinations, interpretations and patterns.

The results may briefly be summarized as follows: 1) most of the students use evolutionary terminology and 2) are fairly good at presenting their thoughts within the theoretical framework in everyday language, although 3) they often show problems in distinguishing processes on molecular, individual, and population levels.

Place, publisher, year, edition, pages
Libreriauniversitaria.it, 2015
National Category
Didactics
Identifiers
urn:nbn:se:sh:diva-28282 (URN)978-88-6292-600-3 (ISBN)
Conference
New perspectives in science education, 4th ed, Florence, March 20-21, 2015.
Available from: 2015-09-18 Created: 2015-09-18 Last updated: 2016-10-03Bibliographically approved
Reyhanian, N., Volkova, K., Hallgren, S., Bollner, T., Olsson, P.-E., Olsén, K. H. & Porsch Hällström, I. (2011). 17α-Ethinyl estradiol affects anxiety and shoaling behavior in adult male zebra fish (Danio rerio). Aquatic Toxicology, 105, 41-48
Open this publication in new window or tab >>17α-Ethinyl estradiol affects anxiety and shoaling behavior in adult male zebra fish (Danio rerio)
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2011 (English)In: Aquatic Toxicology, ISSN 0166-445X, E-ISSN 1879-1514, Vol. 105, p. 41-48Article in journal (Refereed) Published
Keywords
Endocrine disruption, Behavior, Zebra fish, Anxiety, Shoaling, Ethinyl estradiol
National Category
Biological Sciences
Identifiers
urn:nbn:se:sh:diva-9406 (URN)10.1016/j.aquatox.2011.05.009 (DOI)000294317500005 ()2-s2.0-79960336976 (Scopus ID)
Available from: 2011-06-22 Created: 2011-06-22 Last updated: 2017-12-11Bibliographically approved
Lilja, K., Prevodnik, A., Gardeström, J., Elfwing, T., Tedengren, M. & Bollner, T. (2008). Regional differences in mRNA responses in blue mussels within the Baltic proper. Comparative Biochemistry and Physiology - Part C: Toxicology & Pharmacology, 148(2), 101-106
Open this publication in new window or tab >>Regional differences in mRNA responses in blue mussels within the Baltic proper
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2008 (English)In: Comparative Biochemistry and Physiology - Part C: Toxicology & Pharmacology, ISSN 1532-0456, E-ISSN 1878-1659, Vol. 148, no 2, p. 101-106Article in journal (Refereed) Published
Abstract [en]

Mussels (Mytilus sp.) from two regions along the permanent salinity gradient within the Baltic proper were exposed to copper (35 ppb) or petrol (0.3 mL/L) for 10 days and analyzed for mRNA expressions in gill tissue. Expression of mRNAs for the heat shock proteins HSP70 and HSP90 was significantly induced by copper, but not by petrol. For the metallothioneins MT10 and MT20, regional differences in mRNA expressions could be seen. In mussels from the northern Baltic proper, MT20 expression increased 2.8 and 3.4 times, after exposure to copper and petrol, respectively. In contrast, no change could be seen in MT20 expression for mussels from the southern Baltic proper. MT10 showed a peculiar expression not previously described. For some mussels, no expression at all was detected, some showed a weak expression and for some individuals a strong expression could be seen. For the mussels from the southern Baltic proper, the number of individuals with a strong expression of MT10 increased from 1 out of 18 (control), to 7 and 8, after exposure to copper and petrol, respectively. The results clearly show that responses vary between different regions within the Baltic proper, which emphasises the importance to study interactions between contaminants, populations and regions.

National Category
Natural Sciences
Identifiers
urn:nbn:se:sh:diva-5823 (URN)10.1016/j.cbpc.2008.04.001 (DOI)000257973400001 ()2-s2.0-45949098210 (Scopus ID)
Available from: 2011-01-31 Created: 2011-01-31 Last updated: 2017-12-11Bibliographically approved
Prevodnik, A., Lilja, K. & Bollner, T. (2007). Benzo[a]pyrene up-regulates the expression of the proliferating cell nuclear antigen (PCNA) and multixenobiotic resistance polyglycoprotein (P-gp) in Baltic Sea blue mussels (Mytilus edulis L.). Comparative Biochemistry and Physiology - Part C: Toxicology & Pharmacology, 145(2), 265-274
Open this publication in new window or tab >>Benzo[a]pyrene up-regulates the expression of the proliferating cell nuclear antigen (PCNA) and multixenobiotic resistance polyglycoprotein (P-gp) in Baltic Sea blue mussels (Mytilus edulis L.)
2007 (English)In: Comparative Biochemistry and Physiology - Part C: Toxicology & Pharmacology, ISSN 1532-0456, E-ISSN 1878-1659, Vol. 145, no 2, p. 265-274Article in journal (Refereed) Published
Abstract [en]

The expression of protein biomarkers in Baltic Sea blue mussels was analyzed after three days exposure to low (2.8 mu g/animal/day), intermediate (28 mu g/animal/day), or high (280 mu g/animal/day) nominal doses of benzo[a]pyrene (BaP). Significant expression changes were found in the animals exposed to the low dose, the lowest reported dose for DNA adduct formation in the gills of Baltic Sea blue mussels. Up-regulated expression of the proliferating cell nuclear antigen (PCNA), quantified from Western blots, and no change in the 5-bromo-deoxyuridine (BrdU) staining pattern, determined by immunocytochemistry, indicated that the observed PCNA response was mainly non-proliferative, and thus possibly due to DNA damage. The expression of the multixenobiotic resistance polyglycoprotein (P-gp) was also up-regulated, proving its usefulness as an exposure marker to planar organic compounds. No effect of the BaP treatment with respect to the retinoblastoma 110 protein or heat shock proteins 60 and 70 was found. The variance in the medium and high dose data was too large to allow for the detection of significant expression changes. We suggest PCNA to be a marker for genotoxic stress derived from the polycyclic aromatic hydrocarbon BaP, irrespective of whether the stress leads to DNA repair or to cell proliferation.

National Category
Natural Sciences
Identifiers
urn:nbn:se:sh:diva-5825 (URN)10.1016/j.cbpc.2006.12.014 (DOI)000245499100012 ()17306628 (PubMedID)2-s2.0-33847409768 (Scopus ID)
Available from: 2011-01-31 Created: 2011-01-31 Last updated: 2017-12-11Bibliographically approved
Prevodnik, A., Gardeström, J., Lilja, K., Elfwing, T., McDonagh, B., Petrovic, N., . . . Bollner, T. (2007). Oxidative stress in response to xenobiotics in the blue mussel Mytilus edulis L.: evidence for variation along a natural. Aquatic Toxicology, 82(1), 63-71
Open this publication in new window or tab >>Oxidative stress in response to xenobiotics in the blue mussel Mytilus edulis L.: evidence for variation along a natural
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2007 (English)In: Aquatic Toxicology, ISSN 0166-445X, E-ISSN 1879-1514, Vol. 82, no 1, p. 63-71Article in journal (Refereed) Published
Abstract [en]

Blue mussels (Mytilus edulis L.) collected at three sampling sites in each of three geographical regions (South, Middle, North) along the permanent longitudinal South-North salinity gradient of the Baltic Sea, were exposed for 10 days to copper (35 ppb) or 95 octane petrol (0.3%0). During the experiment, they were maintained at the respective sampling site salinity. Scope for growth (SFG) was determined, and biochemical stress markers (protein carbonyl groups, disulfide bond formation, and glutathione transferase (GST), and catalase (CAT) activities) were investigated in gill tissue upon termination of the experiment. Treatment and regional effects for SFG and protein carbonyl groups were all significant for petrol. The largest increase in protein carbonyl groups was observed in the North. Mussels from the southern, more saline (similar to 7%) region had the highest SFG, and displayed the largest SFG decrease in response to treatment, indicating that they had the most energy available for allocation to stress response. They also displayed the least increase in the level of protein carbonyl groups. Mussels from the Northern, less saline (similar to 5%) region had the highest degree of protein carbonyl groups in response to both treatments, and lowest average SFG. Silver stained diagonal gels for samples from one sampling site in South and North, respectively, demonstrated differences in disulfide bond profiles for both stress treatments. There was also a regional difference in the number of protein disulfides observed on diagonal gels. The most diverse protein disulfide response was found in South. No treatment related effects on GST and CAT activities were observed. We suggest that both SFG and protein carbonyl groups show that geographical difference in stress susceptibility, previously established between the North and the Baltic Seas, also apply on a regional scale within the Baltic Sea, along the salinity gradient.

National Category
Natural Sciences
Identifiers
urn:nbn:se:sh:diva-5824 (URN)10.1016/j.aquatox.2007.01.006 (DOI)000246231100007 ()17320983 (PubMedID)2-s2.0-33947322375 (Scopus ID)
Available from: 2011-01-31 Created: 2011-01-31 Last updated: 2017-12-11Bibliographically approved
Prevodnik, A., Lilja, K. & Bollner, T. (2006). Effects of copper and cadmium on protein expression in Baltic sea blue mussels (Mytilus edulis sp.). Marine Environmental Research, 62, S90-S91
Open this publication in new window or tab >>Effects of copper and cadmium on protein expression in Baltic sea blue mussels (Mytilus edulis sp.)
2006 (English)In: Marine Environmental Research, ISSN 0141-1136, E-ISSN 1879-0291, Vol. 62, p. S90-S91Article in journal (Refereed) Published
National Category
Natural Sciences
Identifiers
urn:nbn:se:sh:diva-5826 (URN)doi:10.1016/j.marenvres.2006.04.036 (DOI)000238957800073 ()
Available from: 2011-01-31 Created: 2011-01-31 Last updated: 2017-12-11Bibliographically approved
Nässel, D. R., Mentlein, R., Bollner, T. & Karlsson, A. (2000). Proline-specific dipeptidyl peptidase activity in the cockroach brain and intestine: Partial characterization, distribution, and inactivation of tachykinin-related peptides. Journal of Comparative Neurology, 418(1), 81-92
Open this publication in new window or tab >>Proline-specific dipeptidyl peptidase activity in the cockroach brain and intestine: Partial characterization, distribution, and inactivation of tachykinin-related peptides
2000 (English)In: Journal of Comparative Neurology, ISSN 0021-9967, E-ISSN 1096-9861, Vol. 418, no 1, p. 81-92Article in journal (Refereed) Published
Abstract [en]

Proline-specific dipeptidyl peptidase (DPP TV) is an established enzyme known to degrade neuropeptides and peptide hormones in vertebrate tissues. DPP TV cleaves peptides at the Pro(2) residue. Because several neuropeptides of the cockroach Leucophaea maderae, such as LemTRP-1 (APSGFLGVRamide), are potential substrates for this peptidase, we investigated the occurrence of proline-specific DPP activity in cockroach tissues. Partly purified DPP activity was characterized from the brain and midgut of L. maderae by using Gly-Pro-4-nitroanilide as a substrate. The highest activity was obtained from the membrane fraction of intestine; about 10 times less activity (per milligram protein) was obtained from brain membranes. A smaller amount of soluble DPP activity could also be identified in both tissues. Gel chromatography of the solubilized intestinal DPP activity revealed a molecular mass of about 75 kDa. The enzyme had a pH optimum of 8.5. Diprotin A (Ile-Pro-Ile) was an efficient competitive inhibitor of the cockroach DPP, whereas other known DPP inhibitors were found to be less potent. When incubated with human and cockroach DPP IV, the cleavage products of LemTRP-1 were AP and SGFLGVRamide (des-AP-LemTRP-1) as determined by mass spectrometry of high-performance liquid chromatography (HPLC)-purified peptide fragments. The AP fragment was biologically inactive and the des-AP fragment had a drastically reduced myostimulatory activity on the hindgut of L. maderae. The blowfly TRP callitachykinin-I (CavTK-I; APTAFYGVRamide) was cleaved in two steps to des-AP-CavTK-I and desAPTA-CavTK-I, showing that cockroach DPP does not only liberate Xaa-Pro, but also Xaa-Ala dipeptides. The fragment desAPTA-CavTK-I was completely inactive on the cockroach hindgut. To compare, LemTRP-3 and CavTK-II, which lack a Pro(2), were not cleaved by DPP IV. Enzyme histochemistry for DPP IV was performed on cryostat sections of brain and intestine with Gly-Pro-4-methoxy-2-naphthylamide as the substrate and Fast Blue B as the chromogen. Strong histochemical labeling was seen in specific neuropils of the brain such as the calyces of the mushroom bodies, the antennal glomeruli, and the central body. Also, the inner lining of the midgut (the peritrophic membrane) and the malpighian tubules were strongly labeled by reaction product. In both the brain and intestine, the enzyme-histochemical reaction was inhibited by diprotin A.

National Category
Natural Sciences
Identifiers
urn:nbn:se:sh:diva-5827 (URN)10.1002/(SICI)1096-9861(20000228)418:1<81::AID-CNE6>3.0.CO;2-B (DOI)000085189600006 ()
Available from: 2011-01-31 Created: 2011-01-31 Last updated: 2017-12-11Bibliographically approved
Porseryd, T., Larsson, J., Kellner, M., Bollner, T., Dinnétz, P. & Porsch Hällström, I.Altered non-reproductive behavior and feminization caused by developmental exposure to 17α-ethinylestradiol persist to adulthood in three-spined stickleback (Gasterosteus aculeatus).
Open this publication in new window or tab >>Altered non-reproductive behavior and feminization caused by developmental exposure to 17α-ethinylestradiol persist to adulthood in three-spined stickleback (Gasterosteus aculeatus)
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(English)Manuscript (preprint) (Other academic)
National Category
Environmental Sciences
Research subject
Environmental Studies
Identifiers
urn:nbn:se:sh:diva-34932 (URN)
Funder
The Foundation for Baltic and East European Studies
Note

As manuscript in dissertation.

Available from: 2018-05-04 Created: 2018-05-04 Last updated: 2018-05-04Bibliographically approved
Prevodnik, A., Lilja, K. & Bollner, T.Protein biomarker response in Baltic Sea blue mussels (Mytilus edulis L.) exposed to copper or cadmium.
Open this publication in new window or tab >>Protein biomarker response in Baltic Sea blue mussels (Mytilus edulis L.) exposed to copper or cadmium
(English)Manuscript (preprint) (Other academic)
National Category
Biological Sciences
Identifiers
urn:nbn:se:sh:diva-31467 (URN)
Note

Som manuskript i avhandling. As manuscript in dissertation.,

Available from: 2007-03-29 Created: 2016-12-20 Last updated: 2016-12-20Bibliographically approved
Prevodnik, A., Skarphéðinsdóttir, H., Ericson, G. & Bollner, T.Tidal impact on pollutant stress responses in Icelandic blue mussels (Mytilus edulis L.).
Open this publication in new window or tab >>Tidal impact on pollutant stress responses in Icelandic blue mussels (Mytilus edulis L.)
(English)Manuscript (preprint) (Other academic)
National Category
Biological Sciences
Identifiers
urn:nbn:se:sh:diva-31468 (URN)
Note

Som manuskript i avhandling. As manuscript in dissertation.

Available from: 2007-03-29 Created: 2016-12-20 Last updated: 2016-12-20Bibliographically approved
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