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Olsén, K. Håkan
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Publications (10 of 49) Show all publications
Olsén, K. H., Sukovich, N., Backman, J. & Lundh, T. (2018). Chemical foraging stimulation in the omnivorous species crucian carp, Carassius carassius (Linnaeus 1758). Aquaculture Reports, 12, 36-42
Open this publication in new window or tab >>Chemical foraging stimulation in the omnivorous species crucian carp, Carassius carassius (Linnaeus 1758)
2018 (English)In: Aquaculture Reports, ISSN 2352-5134, Vol. 12, p. 36-42Article in journal (Refereed) Published
Abstract [en]

In fish aquaculture, great efforts are made to develop feed that excludes marine fish proteins and oils, or at least to decrease these parts to a minimum but the fish might not be attracted to or might not eat the alternative feed. Molecules detected by the olfactory sense can induce arousal and search behavior that attract the fish and induce foraging. In the present study, we have examined whether mussel extracts can induce foraging behavior in crucian carp, Carassius carassius. Free amino acids are present in high concentrations in mussels and they are detected at low concentrations by olfaction and induce food search behavior in several fish species. We studied the behavior responses to water extracts of mussel powder compared to extracts of a plant-based commercial carp feed. Extracts of different concentrations were administered in glass aquaria with individual fish. The tests began with 5 min with only tap water and this was followed by 5 min with extracts or amino acid solutions. The behavior was recorded with a video camera. The initial control period was compared to the following period with odors. No significant foraging response was observed with extracts of mussels, but the commercial carp feed extract induced increased bottom snapping. Synthetic mixtures of five nutritional amino acids present at high concentrations in both extracts did not give any significant foraging response. Our results show that mussels are not suitable as a protein source in feed to crucian carp. 

Place, publisher, year, edition, pages
Elsevier B.V., 2018
Keywords
Bottom snapping, Carp feed extracts, Foraging behavior, Mussel meal extracts, Olfactory sense
National Category
Biological Sciences
Identifiers
urn:nbn:se:sh:diva-36504 (URN)10.1016/j.aqrep.2018.09.003 (DOI)2-s2.0-85053816755 (Scopus ID)
Funder
Stockholm County CouncilThe Foundation for Baltic and East European Studies, A07-09
Available from: 2018-10-09 Created: 2018-10-09 Last updated: 2018-10-10Bibliographically approved
Kellner, M., Porseryd, T., Porsch Hällström, I., Borg, B., Roufidou, C. & Olsén, K. H. (2018). Developmental exposure to the SSRI citalopram causes long-lasting behavioural effects in the three-spined stickleback (Gasterosteus aculeatus). Ecotoxicology, 27(1), 12-22
Open this publication in new window or tab >>Developmental exposure to the SSRI citalopram causes long-lasting behavioural effects in the three-spined stickleback (Gasterosteus aculeatus)
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2018 (English)In: Ecotoxicology, ISSN 0963-9292, E-ISSN 1573-3017, Vol. 27, no 1, p. 12-22Article in journal (Refereed) Published
Abstract [en]

Selective Serotonin Re-uptake Inhibitors (SSRIs) are a class of psychotropic drugs used to treat depression in both adolescents and pregnant or breast-feeding mothers as well as in the general population. Recent research on rodents points to persistent behavioural effects of pre- and perinatal exposure to SSRI which last into adulthood. To study effects of developmental exposure in fish, three-spine sticklebacks were exposed to 1.5 µg/l of the SSRI citalopram in the ambient water for 30 days, starting two days post-fertilisation. After 100 days of remediation in clean water the fish were put through an extensive test battery. Feeding behaviour was tested as the number of bites against a piece of food and found to be increased in the exposed fish. Aggression levels were measured as the number of bites against a mirror image during 10 minutes and was also found to be significantly increased in the exposed fish. Novel tank behaviour and locomotor activity was tested in an aquarium that had a horizontal line drawn half-way between the bottom and the surface. Neither the latency to the first transition to the upper half, nor the number of transitions or the total time spent in the upper half was affected by treatment. Locomotor activity was significantly reduced in the exposed fish. The light/dark preference was tested in an aquarium where the bottom and walls were black on one side and white on the other. The number of transitions to the white side was significantly reduced in the exposed fish but there was no effect on the latency to the first transition or the total time spent in the white half. The results in the current study indicate that developmental SSRI exposure causes persistent behavioural effects in fish and contribute to the existing knowledge about SSRIs as environmental pollutants.

Keywords
Aggression; Feeding; Fish; Locomotor; SSRI; Scototaxis
National Category
Environmental Sciences
Research subject
Environmental Studies
Identifiers
urn:nbn:se:sh:diva-32426 (URN)10.1007/s10646-017-1866-4 (DOI)000419679500003 ()29058178 (PubMedID)2-s2.0-85031892443 (Scopus ID)
Funder
The Foundation for Baltic and East European Studies, 1352/3.1.1/2015Stockholm County Council, 806/3.1.1/2014
Note

As manuscript in dissertation. with title: Developmental exposure to the SSRI citalopram causes persistent behavioural effects in the three-spined stickleback (Gasterosteus aculeatus)

Available from: 2017-04-19 Created: 2017-04-19 Last updated: 2018-07-05Bibliographically approved
Nielsen, S. V., Kellner, M., Henriksen, P. G., Olsén, H., Hansen, S. H. & Baatrup, E. (2018). The psychoactive drug Escitalopram affects swimming behaviour and increases boldness in zebrafish (Danio rerio). Ecotoxicology, 27(4), 485-497
Open this publication in new window or tab >>The psychoactive drug Escitalopram affects swimming behaviour and increases boldness in zebrafish (Danio rerio)
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2018 (English)In: Ecotoxicology, ISSN 0963-9292, E-ISSN 1573-3017, Vol. 27, no 4, p. 485-497Article in journal (Refereed) Published
Abstract [en]

was not significantly different from control fish in either sex. The results of this study demonstrate that Escitalopram can affect subtle but ecologically important aspects of fish behaviour and lends further credibility to the assumption that Escitalopram is an environmentally active pharmaceutical.

Keywords
Altered swimming behaviour, Escitalopram, Increased boldness, Psychoactive drug, SSRI, Zebrafish
National Category
Environmental Sciences
Identifiers
urn:nbn:se:sh:diva-34808 (URN)10.1007/s10646-018-1920-x (DOI)000429932600010 ()29541889 (PubMedID)2-s2.0-85043691062 (Scopus ID)
Funder
The Foundation for Baltic and East European StudiesStockholm County Council
Available from: 2018-03-29 Created: 2018-03-29 Last updated: 2018-07-05Bibliographically approved
Porseryd, T., Kellner, M., Reyhanian, N., Volkova, K., Elabbas, L., Ullah, S., . . . Porsch Hällström, I. (2017). Combinatory effects of low concentrations of 17α-etinylestradiol and citalopram on non-reproductive behavior in adult zebrafish (Danio rerio). Aquatic Toxicology, 193, 9-17
Open this publication in new window or tab >>Combinatory effects of low concentrations of 17α-etinylestradiol and citalopram on non-reproductive behavior in adult zebrafish (Danio rerio)
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2017 (English)In: Aquatic Toxicology, ISSN 0166-445X, E-ISSN 1879-1514, Vol. 193, p. 9-17Article in journal (Refereed) Published
Abstract [en]

Sewage treatment plant effluents contain a complex mixture of pharmaceuticals, personal care products and industrial chemicals, thus exposing aquatic organisms. Still, the consequences of exposure to combinations of different classes of drugs is largely unknown. In this study, we expose adult zebrafish (Danio rerio) males and females to low, environmentally relevant concentrations of the endocrine disrupting chemical 17α-ethinyl estradiol (EE2) and the selective serotonin re-uptake inhibitor (SSRI) citalopram, alone and in combination, and analyse three non-reproductive behaviours of importance for population fitness.

Two weeks exposure to 0.1 and 0.5 ng/LEE2 resulted in increased anxiety in males in the scototaxis (light/dark preference) test. Significantly longer latency periods before entering the white zone and fewer visits in the white zone were observed in males exposed to both 0.1 and 0.5 ng/LEE2 compared to unexposed males. No significant effects of citalopram alone (0.1 and 0.5 µg/L) were observed in the scototaxis test. The combined exposures (0.1 ng/L EE2 + 0.1 µg/L citalopram and 0.5 ng/L EE2 + 0.5 µg/L citalopram) resulted in abolishment of the anxiogenic effects of EE2, with significantly shorter latency period (low dose) and more transitions to white (high and low dose) than in fish exposed to EE2 alone. No significant effects of either EE2, citalopramor the combination of the two were observed in females. In the novel tank test, significantly more transitions to the upper half of the tank were observed in males exposed to 0.1 µg/L citalopram alone compared to unexposed males while males exposed to 0.1 ng/lEE2 had significantly shorter latency period to enter the upper half. Exposure to the combination of the two low concentrations did, however, result in a significantly longer latency and fewer transitions to upper half compared to both control, EE2- and citalopram-exposed males. These males also spent significantly less time in the upper half than the fish exposed to 0.1 ng/l EE2 or 0.1 µg/l citalopram alone. No significant effects on novel tank behaviour were observed in females or males exposed to the higher concentrations. In the shoaling test, males exposed to 0.1 µg/L citalopram and females exposed to 0.5 ng/l EE2 made significantly fewer transitions away from peers while males exposed to 0.1 µg/L citalopram + 0.1 ng/l EE2 performed significantly more transitions than the fish exposed to 0.1 µg/L citalopram alone.

In conclusion, this study shows that very low concentrations ofEE2, at or slightly above the predicted noeffect concentration (NOEC), affects anxiety in zebrafish males. Furthermore, citalopram, in spite of marginal effect of its own at such low levels, counteracts the response to EE2. This study represents an initial effort to understand the effects on water-living organisms of the cocktails of anthropogenic substances contaminating aquatic environments.

National Category
Environmental Sciences
Research subject
Environmental Studies
Identifiers
urn:nbn:se:sh:diva-32427 (URN)10.1016/j.aquatox.2017.10.001 (DOI)000417658800002 ()29017090 (PubMedID)2-s2.0-85030678324 (Scopus ID)
Funder
Stockholm County Council, 806/3.1.1/2014The Foundation for Baltic and East European Studies, 1352/3.1.1/2015
Note

As manuscript in dissertation with title: Combinatory effects of low-dose 17alpha-etinyl estradiol and citalopram on behavior in adult zebrafish (Danio rerio)

Available from: 2017-04-19 Created: 2017-04-19 Last updated: 2018-04-30Bibliographically approved
Olsén, K. H. & Lundh, T. (2017). Feeding stimulants in an omnivorous species, crucian carp Carassius carassius (Linnaeus 1758). Paper presented at 26th Annual Meeting of the European-Chemoreception-Research-Organization (ECRO), SEP 07-10, 2016, Athens, GREECE. Chemical Senses, 42(2), E46-E47
Open this publication in new window or tab >>Feeding stimulants in an omnivorous species, crucian carp Carassius carassius (Linnaeus 1758)
2017 (English)In: Chemical Senses, ISSN 0379-864X, E-ISSN 1464-3553, Vol. 42, no 2, p. E46-E47Article in journal, Meeting abstract (Other academic) Published
National Category
Environmental Sciences
Identifiers
urn:nbn:se:sh:diva-32357 (URN)000397065700107 ()
Conference
26th Annual Meeting of the European-Chemoreception-Research-Organization (ECRO), SEP 07-10, 2016, Athens, GREECE
Available from: 2017-04-10 Created: 2017-04-10 Last updated: 2017-11-29Bibliographically approved
Sommer, C. & Olsén, K. H. (2017). Sex odour preference in guppy (Poecilia wingei) males are influenced by the social environment. Paper presented at 26th Annual Meeting of the European-Chemoreception-Research-Organization (ECRO), SEP 07-10, 2016, Athens, GREECE. Chemical Senses, 42(2), E47-E47
Open this publication in new window or tab >>Sex odour preference in guppy (Poecilia wingei) males are influenced by the social environment
2017 (English)In: Chemical Senses, ISSN 0379-864X, E-ISSN 1464-3553, Vol. 42, no 2, p. E47-E47Article in journal, Meeting abstract (Other academic) Published
National Category
Environmental Sciences
Identifiers
urn:nbn:se:sh:diva-32358 (URN)000397065700108 ()
Conference
26th Annual Meeting of the European-Chemoreception-Research-Organization (ECRO), SEP 07-10, 2016, Athens, GREECE
Available from: 2017-04-10 Created: 2017-04-10 Last updated: 2017-11-29Bibliographically approved
Jeffries, D. L., Copp, G. H., Lawson Handley, L., Olsén, K. H., Sayer, C. D. & Hänfling, B. (2016). Comparing RADseq and microsatellites to infer complex phylogeographic patterns, an empirical perspective in the Crucian carp, Carassius carassius, L. Molecular Ecology, 25(13), 2997-3018
Open this publication in new window or tab >>Comparing RADseq and microsatellites to infer complex phylogeographic patterns, an empirical perspective in the Crucian carp, Carassius carassius, L
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2016 (English)In: Molecular Ecology, ISSN 0962-1083, E-ISSN 1365-294X, Vol. 25, no 13, p. 2997-3018Article in journal (Refereed) Published
Abstract [en]

The conservation of threatened species must be underpinned by phylogeographic knowledge. This need is epitomised by the freshwater fish Carassius carassius, which is in decline across much of its European range. Restriction site associated DNA sequencing (RADseq) is increasingly used for such applications, however RADseq is expensive, and limitations on sample number must be weighed against the benefit of large numbers of markers. This trade-off has previously been examined using simulation studies, however, empirical comparisons between these markers, especially in a phylogeographic context, are lacking. Here, we compare the results from microsatellites and RADseq for the phylogeography of C. carassius to test whether it is more advantageous to genotype fewer markers (microsatellites) in many samples, or many markers (SNPs) in fewer samples. These datasets, along with data from the mitochondrial cytochrome b gene, agree on broad phylogeographic patterns; showing the existence of two previously unidentified C. carassius lineages in Europe; one found throughout northern and central-eastern European drainages, and a second almost exclusively confined to the Danubian catchment. These lineages have been isolated for approximately 2.15 M years, and should be considered separate conservation units. RADseq recovered finer population structure and stronger patterns of IBD than microsatellites, despite including only 17.6% of samples (38% of populations and 52% of samples per population). RADseq was also used along with Approximate Bayesian Computation to show that the postglacial colonisation routes of C. carassius differ from the general patterns of freshwater fish in Europe, likely as a result of their distinctive ecology.

National Category
Biological Sciences
Identifiers
urn:nbn:se:sh:diva-29754 (URN)10.1111/mec.13613 (DOI)000378942200005 ()26971882 (PubMedID)2-s2.0-84970939129 (Scopus ID)
Funder
The Foundation for Baltic and East European Studies
Available from: 2016-03-18 Created: 2016-03-18 Last updated: 2018-04-05Bibliographically approved
Olsén, K. H. & Lundh, T. (2016). Feeding stimulants in an omnivorous species, crucian carp Carassius carassius (Linnaeus 1758). Aquaculture Reports, 4, 66-73
Open this publication in new window or tab >>Feeding stimulants in an omnivorous species, crucian carp Carassius carassius (Linnaeus 1758)
2016 (English)In: Aquaculture Reports, ISSN 2352-5134, Vol. 4, p. 66-73Article in journal (Refereed) Published
Abstract [en]

Many fish are during feeding dependent on both an olfactory and gustatory sense. Olfaction that acts as the distance sense induces arousal, food search behaviour and attraction to the source, followed by examination of food items by the gustatory sense. During buccal handling the fish decide if the feed will be rejected or swallowed. Amino acids are often stimulatory to the gustatory sense and can act as feeding stimulants. There are, however, inter-species differences concerning what kinds of amino acids act as feeding stimulants or deterrents. The species differences are probably dependent on the natural food choice. As feeding stimulating molecules increase feeding and growth, but deterrents have the reverse effect, it is important to know what kind of molecules have either effect. In the present study we record mouth handling time in the omnivorous crucian carp, Carassius carassius, of agar pellets containing water extracts of meal consisting of ordinary food pellets, blue mussels or a commercial carp attractant. These tests were followed by testing with agar pellets with synthetic amino acids, based on the content of the water extracts of the food pellets that was the only feeding stimulant. Neither extracts of mussel meal or of commercial carp attractants had a stimulating effect, i.e. no significant difference in handling time compared to agar pellets with only water. A mixture of five of the major amino acids in the food pellet extract (40 mM alanine, 20 mM glycine, 20 mM arginine, 8 mM serine, 8 mM leucin) gave a significant longer handling time compared to agar pellets with only water. The handling time was also longer for the three amino acids that had the highest concentrations (40 mM Ala, 20 mM Gly, 20 mM Arg) and finally with only alanine (128 mM). Agar pellets with only Ala gave, however, a significant shorter handling time compared to agar pellets with food pellet extract. The mussel meal extract had the same content of free amino acids and their ranking order was the same as in extracts of food pellets, but at much higher concentrations. Based on the free amino acid content, the mussel extract should have stimulated feeding. This indicates that the mussel extract contained compounds that acted as feeding deterrents in omnivorous crucian carp that do not feed on blue mussels in their natural environment. Previous studies have shown that blue mussel extracts act as feeding stimulants in several bottom feeding carnivorous fish. We finally tested betaine (100 mM) but the molecule had no significant stimulating effect that has been observed in some other fish species. © 2016 The Authors

Keywords
Agar pellets, Feed extracts, Feeding stimulants, Free amino acids, Gustatory sense, Mouth handling time, Mussel meal
National Category
Biological Sciences
Research subject
Environmental Studies; Baltic and East European studies
Identifiers
urn:nbn:se:sh:diva-30641 (URN)10.1016/j.aqrep.2016.06.005 (DOI)2-s2.0-84977604199 (Scopus ID)1302/42/2009 (Local ID)1302/42/2009 (Archive number)1302/42/2009 (OAI)
Projects
The story of crucian carp (Carassius carassius) in the Baltic Sea region - History and a possible future
Funder
The Foundation for Baltic and East European Studies, A017-09Stockholm County Council
Available from: 2016-07-20 Created: 2016-07-19 Last updated: 2018-10-10Bibliographically approved
Bonow, M., Olsén, H. & Svanberg, I. (Eds.). (2016). Historical Aquaculture in Northern Europe (1ed.). Huddinge: Södertörns högskola
Open this publication in new window or tab >>Historical Aquaculture in Northern Europe
2016 (English)Collection (editor) (Refereed)
Abstract [en]

How were fishponds introduced, farmed and spread in Scandinavia and the Baltic Region in early modern times? What was their economic, social and religious importance? Which fish species were significant and why?

This book uncovers a long, now broken, tradition that barely left traces in the written record or physical environment. Its broad and multidisciplinary scope highlights the situation from medieval times until the late nineteenth century. Besides Scandinavia and the Baltic States, insights from England are also introduced.

Several socio-cultural domains have been identified: late medieval monastic fishponds; late medieval aristocratic fishponds associated with castles and manors; seventeenth and eighteenth century ponds rectory ponds as well as urban ponds from the seventeenth century to the nineteenth century.

Place, publisher, year, edition, pages
Huddinge: Södertörns högskola, 2016. p. 204 Edition: 1
Series
Research Reports, ISSN 1403-5111 ; 2016:1
National Category
Environmental Sciences
Research subject
Environmental Studies; Baltic and East European studies
Identifiers
urn:nbn:se:sh:diva-31251 (URN)1302/42/2009 (Local ID)978-91-87843-62-4 (ISBN)1302/42/2009 (Archive number)1302/42/2009 (OAI)
Projects
The Story of Crucian Carp in the Baltic Sea Region: History and a Possible Future
Funder
The Foundation for Baltic and East European Studies, A017-09
Available from: 2016-12-01 Created: 2016-11-30 Last updated: 2018-08-27Bibliographically approved
Sommer, C. & Olsén, K. H. (2016). Sex odour preference in guppy (Poecilia wingei) males is influenced by the social environment. Behaviour, 153(12), 1419-1434
Open this publication in new window or tab >>Sex odour preference in guppy (Poecilia wingei) males is influenced by the social environment
2016 (English)In: Behaviour, ISSN 0005-7959, E-ISSN 1568-539X, Vol. 153, no 12, p. 1419-1434Article in journal (Refereed) Published
Abstract [en]

The social environment of animals, particularly in the early stages of life, can have great impact on species-specific and sex-specific behaviours. These changes can be irreversible and continue during the entire life. In the present study we asked the question whether the social environment of male Endler's guppies, Poecilia wingei, housed in an all-male community could affect their preference response to female or male odour cues in a flow through Y-maze. After 30 days in an all-male group males were tested for their preference-avoidance responses to conspecific odours. The males were attracted to male-scented water but not to water scented by females. In simultaneous choice between male and female odours they demonstrated no significant preference. The males were attracted to male-scented water after they were kept for 48 h or 12 days with females. After the Y-maze tests the males' were placed with two females and their courting behaviour were recorded. The males showed low frequencies of reproductive behaviours. In the all-male group the males had been courting each other. The results show that the social environment influence sexual odour preference and courting behaviour in guppy males.

Keywords
male guppy, sex odour preference, social environment
National Category
Biological Sciences
Identifiers
urn:nbn:se:sh:diva-31078 (URN)10.1163/1568539X-00003387 (DOI)000385723200004 ()2-s2.0-84992066374 (Scopus ID)
Available from: 2016-11-03 Created: 2016-11-03 Last updated: 2017-11-29Bibliographically approved
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