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Smialowska, Agata
Publikationer (3 of 3) Visa alla publikationer
Kärblane, K., Gerassimenko, J., Nigul, L., Piirsoo, A., Smialowska, A., Vinkel, K., . . . Sarmiento, C. (2015). ABCE1 Is a Highly Conserved RNA Silencing Suppressor. PLOS ONE, 10(2), Article ID e0116702.
Öppna denna publikation i ny flik eller fönster >>ABCE1 Is a Highly Conserved RNA Silencing Suppressor
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2015 (Engelska)Ingår i: PLOS ONE, E-ISSN 1932-6203, Vol. 10, nr 2, artikel-id e0116702Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

ATP-binding cassette sub-family E member 1 (ABCE1) is a highly conserved protein among eukaryotes and archaea. Recent studies have identified ABCE1 as a ribosome-recycling factor important for translation termination in mammalian cells, yeast and also archaea. Here we report another conserved function of ABCE1. We have previously described AtRLI2, the homolog of ABCE1 in the plant Arabidopsis thaliana, as an endogenous suppressor of RNA silencing. In this study we show that this function is conserved: human ABCE1 is able to suppress RNA silencing in Nicotiana benthamiana plants, in mammalian HEK293 cells and in the worm Caenorhabditis elegans. Using co-immunoprecipitation and mass spectrometry, we found a number of potential ABCE1-interacting proteins that might support its function as an endogenous suppressor of RNA interference. The interactor candidates are associated with epigenetic regulation, transcription, RNA processing and mRNA surveillance. In addition, one of the identified proteins is translin, which together with its binding partner TRAX supports RNA interference.

Nationell ämneskategori
Biologiska vetenskaper
Identifikatorer
urn:nbn:se:sh:diva-26400 (URN)10.1371/journal.pone.0116702 (DOI)000349444900060 ()25659154 (PubMedID)2-s2.0-84922720162 (Scopus ID)
Forskningsfinansiär
Östersjöstiftelsen, 300501
Tillgänglig från: 2015-02-12 Skapad: 2015-02-12 Senast uppdaterad: 2021-06-14Bibliografiskt granskad
Smialowska, A., Djupedal, I., Wang, J., Kylsten, P., Swoboda, P. & Ekwall, K. (2014). RNAi mediates post-transcriptional repression of gene expression in fission yeast Schizosaccharomyces pombe. Biochemical and Biophysical Research Communications - BBRC, 444(2), 254-259
Öppna denna publikation i ny flik eller fönster >>RNAi mediates post-transcriptional repression of gene expression in fission yeast Schizosaccharomyces pombe
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2014 (Engelska)Ingår i: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 444, nr 2, s. 254-259Artikel i tidskrift (Övrigt vetenskapligt) Published
Abstract [en]

RNA interference (RNAi) is a gene silencing mechanism conserved from fungi to mammals. Small interfering RNAs are products and mediators of the RNAi pathway and act as specificity factors in recruiting effector complexes. The Schizosaccharomyces pombe genome encodes one of each of the core RNAi proteins, Dicer, Argonaute and RNA-dependent RNA polymerase (dcr1, ago1, rdp1). Even though the function of RNAi in heterochromatin assembly in S. pombe is established, its role in controlling gene expression is elusive. Here, we report the identification of small RNAs mapped anti-sense to protein coding genes in fission yeast. We demonstrate that these genes are up-regulated at the protein level in RNAi mutants, while their mRNA levels are not significantly changed. We show that the repression by RNAi is not a result of heterochromatin formation. Thus, we conclude that RNAi is involved in post-transcriptional gene silencing in S. pombe.

Nyckelord
Fission yeast, PTGS, RNAi
Nationell ämneskategori
Biokemi och molekylärbiologi
Identifikatorer
urn:nbn:se:sh:diva-22232 (URN)10.1016/j.bbrc.2014.01.057 (DOI)000331923500025 ()24462781 (PubMedID)2-s2.0-84892926000 (Scopus ID)
Forskningsfinansiär
Östersjöstiftelsen, 300501Cancerfonden, CAN-2009Vetenskapsrådet, VR-NT-2007-4722
Tillgänglig från: 2014-02-14 Skapad: 2014-02-14 Senast uppdaterad: 2018-07-20Bibliografiskt granskad
Rhind, N., Chen, Z., Yassour, M., Thompson, D. A., Haas, B. J., Habib, N., . . . Nusbaum, C. (2011). Comparative Functional Genomics of the Fission Yeasts. Science, 332(6032), 930-936
Öppna denna publikation i ny flik eller fönster >>Comparative Functional Genomics of the Fission Yeasts
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2011 (Engelska)Ingår i: Science, ISSN 0036-8075, E-ISSN 1095-9203, Vol. 332, nr 6032, s. 930-936Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The fission yeast clade-comprising Schizosaccharomyces pombe, S. octosporus, S. cryophilus, and S. japonicus-occupies the basal branch of Ascomycete fungi and is an important model of eukaryote biology. A comparative annotation of these genomes identified a near extinction of transposons and the associated innovation of transposon-free centromeres. Expression analysis established that meiotic genes are subject to antisense transcription during vegetative growth, which suggests a mechanism for their tight regulation. In addition, trans-acting regulators control new genes within the context of expanded functional modules for meiosis and stress response. Differences in gene content and regulation also explain why, unlike the budding yeast of Saccharomycotina, fission yeasts cannot use ethanol as a primary carbon source. These analyses elucidate the genome structure and gene regulation of fission yeast and provide tools for investigation across the Schizosaccharomyces clade.

Nationell ämneskategori
Biokemi och molekylärbiologi Mikrobiologi
Identifikatorer
urn:nbn:se:sh:diva-14957 (URN)10.1126/science.1203357 (DOI)000290766600034 ()21511999 (PubMedID)2-s2.0-79956319465 (Scopus ID)
Tillgänglig från: 2012-01-24 Skapad: 2012-01-24 Senast uppdaterad: 2018-07-18Bibliografiskt granskad
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