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Kylsten, Per
Publikasjoner (10 av 14) Visa alla publikasjoner
Kärblane, K., Gerassimenko, J., Nigul, L., Piirsoo, A., Smialowska, A., Vinkel, K., . . . Sarmiento, C. (2015). ABCE1 Is a Highly Conserved RNA Silencing Suppressor. PLOS ONE, 10(2), Article ID e0116702.
Åpne denne publikasjonen i ny fane eller vindu >>ABCE1 Is a Highly Conserved RNA Silencing Suppressor
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2015 (engelsk)Inngår i: PLOS ONE, E-ISSN 1932-6203, Vol. 10, nr 2, artikkel-id e0116702Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

ATP-binding cassette sub-family E member 1 (ABCE1) is a highly conserved protein among eukaryotes and archaea. Recent studies have identified ABCE1 as a ribosome-recycling factor important for translation termination in mammalian cells, yeast and also archaea. Here we report another conserved function of ABCE1. We have previously described AtRLI2, the homolog of ABCE1 in the plant Arabidopsis thaliana, as an endogenous suppressor of RNA silencing. In this study we show that this function is conserved: human ABCE1 is able to suppress RNA silencing in Nicotiana benthamiana plants, in mammalian HEK293 cells and in the worm Caenorhabditis elegans. Using co-immunoprecipitation and mass spectrometry, we found a number of potential ABCE1-interacting proteins that might support its function as an endogenous suppressor of RNA interference. The interactor candidates are associated with epigenetic regulation, transcription, RNA processing and mRNA surveillance. In addition, one of the identified proteins is translin, which together with its binding partner TRAX supports RNA interference.

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Identifikatorer
urn:nbn:se:sh:diva-26400 (URN)10.1371/journal.pone.0116702 (DOI)000349444900060 ()25659154 (PubMedID)2-s2.0-84922720162 (Scopus ID)
Forskningsfinansiär
The Foundation for Baltic and East European Studies, 300501
Tilgjengelig fra: 2015-02-12 Laget: 2015-02-12 Sist oppdatert: 2021-06-14bibliografisk kontrollert
Smialowska, A., Djupedal, I., Wang, J., Kylsten, P., Swoboda, P. & Ekwall, K. (2014). RNAi mediates post-transcriptional repression of gene expression in fission yeast Schizosaccharomyces pombe. Biochemical and Biophysical Research Communications - BBRC, 444(2), 254-259
Åpne denne publikasjonen i ny fane eller vindu >>RNAi mediates post-transcriptional repression of gene expression in fission yeast Schizosaccharomyces pombe
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2014 (engelsk)Inngår i: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 444, nr 2, s. 254-259Artikkel i tidsskrift (Annet vitenskapelig) Published
Abstract [en]

RNA interference (RNAi) is a gene silencing mechanism conserved from fungi to mammals. Small interfering RNAs are products and mediators of the RNAi pathway and act as specificity factors in recruiting effector complexes. The Schizosaccharomyces pombe genome encodes one of each of the core RNAi proteins, Dicer, Argonaute and RNA-dependent RNA polymerase (dcr1, ago1, rdp1). Even though the function of RNAi in heterochromatin assembly in S. pombe is established, its role in controlling gene expression is elusive. Here, we report the identification of small RNAs mapped anti-sense to protein coding genes in fission yeast. We demonstrate that these genes are up-regulated at the protein level in RNAi mutants, while their mRNA levels are not significantly changed. We show that the repression by RNAi is not a result of heterochromatin formation. Thus, we conclude that RNAi is involved in post-transcriptional gene silencing in S. pombe.

Emneord
Fission yeast, PTGS, RNAi
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Identifikatorer
urn:nbn:se:sh:diva-22232 (URN)10.1016/j.bbrc.2014.01.057 (DOI)000331923500025 ()24462781 (PubMedID)2-s2.0-84892926000 (Scopus ID)
Forskningsfinansiär
The Foundation for Baltic and East European Studies, 300501Swedish Cancer Society, CAN-2009Swedish Research Council, VR-NT-2007-4722
Tilgjengelig fra: 2014-02-14 Laget: 2014-02-14 Sist oppdatert: 2018-07-20bibliografisk kontrollert
Rahman, M. & Kylsten, P. (2011). Rhomboid-7 over-expression results in Opa1-like processing and malfunctioning mitochondria. Biochemical and Biophysical Research Communications - BBRC, 414(2), 315-320
Åpne denne publikasjonen i ny fane eller vindu >>Rhomboid-7 over-expression results in Opa1-like processing and malfunctioning mitochondria
2011 (engelsk)Inngår i: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 414, nr 2, s. 315-320Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Rhomboid-7 (rho-7) is a mitochondrial-specific intramembranous protease. The loss-of-function mutation rho-7 results in semi-lethality, while escapers have a reduced lifespan with several neurological disorders [1]. Here we show that general, or CNS-specific expression of rho-7 can rescue the lethality of rho-7. General, or CNS-specific over-expression of rho-7 in otherwise wild-type animals caused semi-lethality, with approximately 50% of the animals escaping this lethality, developing into adults displaying a shortened life span with larval locomotory problem. On a cellular level, over-expression resulted in severe depression of ATP levels and cytochrome c oxidase subunit II mRNA levels, a lowered number of mitochondria in neurons and aggregation of mitochondria in the brain indicating mitochondrial malfunction. Over-expression of rho-7 in developing eye discs resulted in an elevated apoptotic index. In the CNS, elevated levels of rho-7 were accompanied by both isoforms of Opal-like, a dynamin-like GTPase, a mitochondrial component involved in regulating mitochondrial dynamics and function, including apoptosis. Most, but not all, of rho-7 over-expression phenotypes were suppressed by introducing a heterozygous mutation for Opal-like. Our results suggest that rho-7 and Opal-like function in a common molecular pathway affecting mitochondrial function and apoptosis in Drosophila melanogaster.

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Identifikatorer
urn:nbn:se:sh:diva-14944 (URN)10.1016/j.bbrc.2011.09.047 (DOI)000296404800008 ()2-s2.0-80054841370 (Scopus ID)
Tilgjengelig fra: 2012-01-24 Laget: 2012-01-24 Sist oppdatert: 2017-12-08bibliografisk kontrollert
Markovic, M. P., Kylsten, P. & Dushay, M. S. (2009). Drosophila lamin mutations cause melanotic mass formation and lamellocyte differentiation. Molecular Immunology, 46(16), 3245-3250
Åpne denne publikasjonen i ny fane eller vindu >>Drosophila lamin mutations cause melanotic mass formation and lamellocyte differentiation
2009 (engelsk)Inngår i: Molecular Immunology, ISSN 0161-5890, E-ISSN 1872-9142, Vol. 46, nr 16, s. 3245-3250Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

The fruit fly immune system is a valuable model for invertebrate and innate immunity. Cellular immune reactions in Drosophila are of great interest, especially the molecular genetic mechanisms of hemocyte differentiation and the encapsulation of foreign bodies. Here we report that changes in the lamin gene cause melanotic masses. These darkened clusters of cells result from autoimmune-like encapsulation of self-tissue, as shown by the presence in lam larvae of lamellocytes, effector hemocytes that appear in larvae following wounding or parasitization. Lamins thus affect immunity in Drosophila, and lam mutations can serve as genetic tools to dissect cellular immune signaling and effector pathways.

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Identifikatorer
urn:nbn:se:sh:diva-13881 (URN)10.1016/j.molimm.2009.08.003 (DOI)000271175000009 ()19716177 (PubMedID)2-s2.0-70349567465 (Scopus ID)
Tilgjengelig fra: 2011-12-14 Laget: 2011-12-14 Sist oppdatert: 2017-12-08bibliografisk kontrollert
O'Farrell, F. & Kylsten, P. (2008). A mis-expression study of factors affecting Drosophila PNS cell identity. Biochemical and Biophysical Research Communications - BBRC, 370(4), 657-662
Åpne denne publikasjonen i ny fane eller vindu >>A mis-expression study of factors affecting Drosophila PNS cell identity
2008 (engelsk)Inngår i: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 370, nr 4, s. 657-662Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Drosophila PNS sense organs arise from single sensory organ precursor (SOP) cells through a series of asymmetric divisions. In a mis-expression screen for factors affecting PNS development, we identified string and dappled as being important for the proper formation of adult external sensory (ES) organs. string is a G2 regulator. dappled has no described function but is implicated in tumorigenesis. The misexpression effect from string was analysed using timed over expression during adult ES-organ and, for comparison, embryonic Chorclotonal (Ch) organ formation. Surprisingly, string mis-expression prior to SOP division gave the greatest effect in both systems. In adult ES-organs, this lead to cell fate transformations producing structural cells, whilst in the embryo organs were lost, hence differences within the lineages exist. Mis-expression of dappled, lead to loss and duplications of entire organs in both systems, potentially affecting SOP specification, in addition to affecting neuronal guidance.

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Identifikatorer
urn:nbn:se:sh:diva-14146 (URN)10.1016/j.bbrc.2008.04.030 (DOI)000255883900025 ()18420029 (PubMedID)2-s2.0-43049164296 (Scopus ID)
Merknad

Som manuskript i avhandling. As manuscript in dissertation.

Tilgjengelig fra: 2011-12-18 Laget: 2011-12-16 Sist oppdatert: 2017-12-08bibliografisk kontrollert
O'Farrell, F. & Kylsten, P. (2008). Drosophila Anillin is unequally required during asymmetric cell divisions of the PNS. Biochemical and Biophysical Research Communications - BBRC, 369(2), 407-413
Åpne denne publikasjonen i ny fane eller vindu >>Drosophila Anillin is unequally required during asymmetric cell divisions of the PNS
2008 (engelsk)Inngår i: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 369, nr 2, s. 407-413Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

During Drosophila embryogenesis, timely and orderly asymmetric cell divisions ensure the correct number of each cell type that make up the sensory organs of the larval PNS. We report a role of scraps, Drosophila Anillin, during these divisions. Anillin, a constitutive member of the contractile ring is essential for cytokinesis in Drosophila and vertebrates. During embryogenesis we find that zygotically transcribed scraps is required specifically for the unequal cell divisions, those in which cytokinesis occurs in an "off-centred" manner, of the pIIb and pIIIb neuronal precursor cells, but not the equal cell divisions of the lineage related precursor cells. Complementation and genetic rescue studies demonstrate this effect results from zygotic scraps and leads to polyploidy, ectopic mitosis, and loss of the neuronal precursor daughter cells. The net result of which is the formation of incomplete sense organs and embryonic lethality.

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Identifikatorer
urn:nbn:se:sh:diva-14151 (URN)10.1016/j.bbrc.2008.02.060 (DOI)000254676900023 ()18295597 (PubMedID)2-s2.0-40949150197 (Scopus ID)
Tilgjengelig fra: 2011-12-18 Laget: 2011-12-16 Sist oppdatert: 2017-12-08bibliografisk kontrollert
O'Farrell, F., Esfahani, S. S., Engström, Y. & Kylsten, P. (2008). Regulation of the Drosophila lin-41 homologue dappled by let-7 reveals conservation of a regulatory mechanism within the LIN-41 subclade. Developmental Dynamics, 237(1), 196-208
Åpne denne publikasjonen i ny fane eller vindu >>Regulation of the Drosophila lin-41 homologue dappled by let-7 reveals conservation of a regulatory mechanism within the LIN-41 subclade
2008 (engelsk)Inngår i: Developmental Dynamics, ISSN 1058-8388, E-ISSN 1097-0177, Vol. 237, nr 1, s. 196-208Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Drosophila Dappled (DPLD) is a member of the RBCC/TRIM superfamily, a protein family involved in numerous diverse processes such as developmental timing and asymmetric cell divisions. DPLD belongs to the LIN-41 subclade, several members of which are micro RNA (miRNA) regulated. We re-examined the LIN-41 subclade members and their relation to other RBCC/TRIMs and dpld paralogs, and identified a new Drosophila muscle specific RBCC/TRIM: Another B-Box Affiliate, ABBA. In silico predictions of candidate miRNA regulators of dpld identified let-7 as the strongest candidate. Overexpression of dpld led to abnormal eye development, indicating that strict regulation of dpld mRNA levels is crucial for normal eye development. This phenotype was sensitive to let-7 dosage, suggesting let-7 regulation of dpld in the eye disc. A cell-based assay verified let-7 miRNA down-regulation of dpld expression by means of its 3'-untranslated region. Thus, dpld seems also to be miRNA regulated, suggesting that miRNAs represent an ancient mechanism of LIN-41 regulation.

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Identifikatorer
urn:nbn:se:sh:diva-14180 (URN)10.1002/dvdy.21396 (DOI)000252386300019 ()18069688 (PubMedID)2-s2.0-38149087481 (Scopus ID)
Tilgjengelig fra: 2011-12-18 Laget: 2011-12-16 Sist oppdatert: 2017-12-08bibliografisk kontrollert
Munoz-Alarcon, A., Pavlovic, M., Wismar, J., Schmitt, B., Eriksson, M., Kylsten, P. & Dushay, M. S. (2007). Characterization of lamin Mutation Phenotypes in Drosophila and Comparison to Human Laminopathies. PLOS ONE, 2(6), e532
Åpne denne publikasjonen i ny fane eller vindu >>Characterization of lamin Mutation Phenotypes in Drosophila and Comparison to Human Laminopathies
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2007 (engelsk)Inngår i: PLOS ONE, E-ISSN 1932-6203, Vol. 2, nr 6, s. e532-Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Lamins are intermediate filament proteins that make up the nuclear lamina, a matrix underlying the nuclear membrane in all metazoan cells that is important for nuclear form and function. Vertebrate A-type lamins are expressed in differentiating cells, while B-type lamins are expressed ubiquitously. Drosophila has two lamin genes that are expressed in A-and B-type patterns, and it is assumed that similarly expressed lamins perform similar functions. However, Drosophila and vertebrate lamins are not orthologous, and their expression patterns evolved independently. It is therefore of interest to examine the effects of mutations in lamin genes. Mutations in the mammalian lamin A/C gene cause a range of diseases, collectively called laminopathies, that include muscular dystrophies and premature aging disorders. We compared the sequences of lamin genes from different species, and we have characterized larval and adult phenotypes in Drosophila bearing mutations in the lam gene that is expressed in the B-type pattern. Larvae move less and show subtle muscle defects, and surviving lam adults are flightless and walk like aged wild-type flies, suggesting that lam phenotypes might result from neuromuscular defects, premature aging, or both. The resemblance of Drosophila lam phenotypes to human laminopathies suggests that some lamin functions may be performed by differently expressed genes in flies and mammals. Such still-unknown functions thus would not be dependent on lamin gene expression pattern, suggesting the presence of other lamin functions that are expression dependent. Our results illustrate a complex interplay between lamin gene expression and function through evolution.

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Identifikatorer
urn:nbn:se:sh:diva-14220 (URN)10.1371/journal.pone.0000532 (DOI)000207451600020 ()2-s2.0-70349566715 (Scopus ID)
Tilgjengelig fra: 2011-12-19 Laget: 2011-12-19 Sist oppdatert: 2021-06-14bibliografisk kontrollert
Gallio, M., Englund, C., Kylsten, P. & Samakovlis, C. (2004). Rhomboid 3 orchestrates Slit-independent repulsion of tracheal branches at the CNS midline. Development, 131(15), 3605-3614
Åpne denne publikasjonen i ny fane eller vindu >>Rhomboid 3 orchestrates Slit-independent repulsion of tracheal branches at the CNS midline
2004 (engelsk)Inngår i: Development, ISSN 0950-1991, E-ISSN 1477-9129, Vol. 131, nr 15, s. 3605-3614Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

EGF-receptor ligands act as chemoattractants for migrating epithelial cells during organogenesis and wound healing. We present evidence that Rhomboid 3/EGF signalling, which originates from the midline of the Drosophila ventral nerve cord, repels tracheal ganglionic branches and prevents them from crossing it. rho3 acts independently from the main midline repellent Slit, and originates from a different sub-population of midline cells: the VUM neurons. Expression of dominant-negative Egfr or Ras induces midline crosses, whereas activation of the Egfr or Ras in the leading cell of the ganglionic branch can induce premature turns away from the midline. This suggests that the level of Egfr intracellular signalling, rather than the asymmetric activation of the receptor on the cell surface, is an important determinant in ganglionic branch repulsion. We propose that Egfr activation provides a necessary switch for the interpretation of a yet unknown repellent function of the midline.

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Identifikatorer
urn:nbn:se:sh:diva-15472 (URN)10.1242/dev.01242 (DOI)000223517400010 ()15229181 (PubMedID)2-s2.0-4444361021 (Scopus ID)
Tilgjengelig fra: 2012-02-21 Laget: 2012-02-20 Sist oppdatert: 2017-12-07bibliografisk kontrollert
Gallio, M., Sturgill, G., Rather, P. & Kylsten, P. (2002). A conserved mechanism for extracellular signaling in eukaryotes and prokaryotes. Proceedings of the National Academy of Sciences of the United States of America, 99(19), 12208-12213
Åpne denne publikasjonen i ny fane eller vindu >>A conserved mechanism for extracellular signaling in eukaryotes and prokaryotes
2002 (engelsk)Inngår i: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 99, nr 19, s. 12208-12213Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Epidermal growth factor receptor (EGFr) is a key mediator of cell communication during animal development and homeostasis. In Drosophila, the signaling event is commonly regulated by the polytopic membrane protein Rhomboid (RHO), which mediates the proteolytic activation of EGFr ligands, allowing the secretion of the active signal. Until very recently, the biochemical function of RHO had remained elusive. It is now believed that Drosophila RHO is the founder member of a previously undescribed family of serine proteases, and that it could be directly responsible for the unusual, intramembranous cleavage of EGFr ligands. Here we show that the function of RHO is conserved in Gram-negative bacteria. AarA, a Providencia stuartii RHO-related protein, is active in Drosophila on the fly EGFr ligands. Vice versa, Drosophila RHO-1 can effectively rescue the bacterium's ability to produce or release the signal that activates density-dependent gene regulation (or quorum sensing). This study provides the first evidence that prokaryotic and eukaryotic RHOs could have a conserved role in cell communication and that their biochemical properties could be more similar than previously anticipated.

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Identifikatorer
urn:nbn:se:sh:diva-15786 (URN)10.1073/pnas.192138799 (DOI)000178187000040 ()12221285 (PubMedID)2-s2.0-0037126036 (Scopus ID)
Tilgjengelig fra: 2012-03-08 Laget: 2012-03-07 Sist oppdatert: 2017-07-19bibliografisk kontrollert
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